California Biotechnology Inc.

Scios Inc.

Scios Nova, Inc.

Stewart A Thompson

S Joseph Tarnowski

Barbara Cordell

Linda C Foster

Judith A Abraham

Kate B Silverness

Jeffrey N Higaki

Edmund G Tischer

Trina A Scheuermann

There are disclosed neurotrophic factors which are capable of being expressed as polypeptides lacking the microheterogeneity associated with a related native-sequence factor, CNTF. Also disclosed are DNA sequences encoding the neurotrophic factors and methods for expressing and recovering the factors as homogeneous polypeptides.

Production of homogeneous truncated CNTF

Pharmaceutical formulations and a method for their preparation are provided. These formulations provide a stabilized basic fibroblast growth factor (bFGF) which is less susceptible to oxidation or metal-induced aggregation by including an amount of a chelating agent effective to stabilize the bFGF.

Formulations for stabilizing fibroblast growth factor

DNA sequences are provided which encode a form of basic fibroblast growth factor lacking one of the alanine residues immediately following the N-terminal methionine residue of the primary translation product. The DNA sequences can be expressed to produce basic fibroblast growth factor having a homogeneous N-terminus.

High level expression of basic fibroblast growth factor having a

This invention relates to a method of isolating and purifying proteins such as fibroblast growth factor using metal chelate affinity column chromatography and without the use of heparin. Also disclosed is a method of recovering bFGF multimers and purifying bFGF using metal chelate affinity column chromatography in the absence of heparin.

Method to purify basic fibroblast growth factor

Treatment of basic fibroblast growth factor (bFGF) with organic disulfides, preferably glutathione disulfide, or with inorganic compounds of similar function results in a bFGF composition of enhanced stability and resistance to multimerization. The resulting stabilized form mimics the chromatographic behavior of bFGF as isolated from bovine pituitary.

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Mountain View, CA, United States of America

Stewart A Thompson