Dow Agrosciences LLC

Sangamo Therapeutics, Inc.

Corteva Agriscience LLC

United States of America, As Represented by the Secretary of Agriculture

Agrigenetics, Inc.

Sangamo Biosciences, Inc.

Steven Robert Webb

Lakshmi Sastry-Dent

W Michael Ainley

Zehui Cao

Shreedharan Sriram

Debra L Camper

Jeffrey C Miller

Dmitry Y Guschin

Jayakumar Pon Samuel

German Carlos Spangenberg

Matthew Hayden

Tim Sawbridge

Matthew J Henry

Narasimha Chary Samboju

Manju Gupta

Joseph F Petolino

William M Ainley

Kerrm Y Yau

John White Forster

Noel Cogan

Frank G Burroughs

Frank Burroughs

David Richard Corbin

Donald J Merlo

Kelley Ann Smith

William Michael Ainley

Lei Zhang

Ning Zhou

Ryan C Blue

Michael G Murray

John Mason

Rebecca Ruth Miles

German Spangenberg

Navin Elango

Steven Lee Evans

Krishna M Madduri

Wei Chen

Gaofeng Lin

Mark Allen Thompson

Janna Mai Armstrong

Yidong Ran

Liu Shen

Daniel Isenegger

Matthew A Simpson

John Forster

Fyodor Urnov

Terry R Wright

Justin Michael Lira

Sandeep Kumar

James Wayne Bing

Andrew E Robinson

Gregory J Cost

Stephen N Novak

Sean Michael Russell

Nicole Arnold

Steven Arnold Thompson

Mary E Welter

Charles A Mihaliak

Pon Samuel

Geoffrey J Letchworth

W Mike Ainley

Michael W Ainley

Kelley A Smith

Nicole L Arnold

Dmitry J Guschin

As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

Optimal soybean loci

As disclosed herein, optimal native genomic loci from maize plants have been identified that represent best sites for targeted insertion of exogenous sequences.

Optimal maize loci

Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.

Methods and compositions for integration of an exogenous sequence within the genome of plants

As disclosed herein, optimal native genomic loci have been identified in dicot plants, such as soybean plants, that represent best sites for targeted insertion of exogenous sequences.

As disclosed herein, optimal native genomic loci have been identified in monocot plants, such as maize plants, that represent best sites for targeted insertion of exogenous sequences.

A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

Engineered landing pads for gene targeting in plants

A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

FAD2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified inA3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

Selectable marker genes

An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5' and 3&#x2032; ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Engineered transgene integration platform (ETIP) for gene targeting and trait stacking

A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

Growing community of inventors

Westfield, IN, United States of America

Steven Robert Webb