Life Technologies Corporation

Steven Riddle

Kurt William Vogel

Thomas Machleidt

Matthew B Robers

Kevin Vedvik

Robert Horton

Kristin Huwiler

Gregory Michaud

Rhonda Newman

Compositions, including antibodies, polypeptides, and organic molecules, kits, and methods for probing molecular interactions (e.g., deubiquination, ubiquination and kinase activity), e.g., using resonance energy transfer (RET) are provided.

Kinase and ubiquination assays

The present invention relates to biosensors. In some embodiments, the biosensors are modified ligand binding molecules. In some embodiments, the modified ligand binding molecule is a phosphate binding protein (PBP). In some embodiments, the modified ligand binding molecules are labeled to be capable of RET, e.g., comprising a donor and acceptor moiety. In some embodiments of the invention, there is a detectable change in RET (e.g., FRET) when the modified ligand binding molecule binds and/or releases the ligand (e.g., phosphate). The invention also provides related methods, reactions and assays.

Sensor proteins and assay methods

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Madison, WI, United States of America

Steven Riddle