Illumina Corporation

Oregon Health & Science University

Steven Norberg

Frank J Steemers

Dmitry K Pokholok

Fan Zhang

Ramesh Ramji

Jeffrey G Mandell

Alex Aravanis

Gordon M Cann

Leila Bazargan

Lena Christiansen

Farnaz Absalan

Andrew C Adey

Ryan Mulqueen

Dimitry K Pokholok

Embodiments of systems, methods, and compositions provided herein relate to methods of simultaneously analyzing multiple analytes in a single sample using a single assay. Some embodiments relate to simultaneous analysis of DNA and RNA in a single sample, for example, to the simultaneous generation of DNA and RNA libraries.

Analysis of multiple analytes using a single assay

Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.

Modulating polymer beads for DNA processing

Embodiments of systems, methods, and compositions provided herein relate to hollow beads encapsulating single cells. Some embodiments include performing multiple co-assays on a single cell encapsulated within a hollow bead, including nucleic acid sequencing, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.

Contiguity particle formation and methods of use

A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

Polynucleotide enrichment using CRISPR-Cas systems

Growing community of inventors

La Mesa, CA, United States of America

Steven Norberg