Illumina Corporation

Novartis Ag

Tufts College

Novartis Corporation

Other

Illumina France Sarl

Steven Mark Barnard

M Shane Bowen

Joseph Berger

Adrian Waldner

Maria Candelaria Rogert Bacigalupo

Mark S Chee

John R Stuelpnagel

David R Walt

Andrew Antony Brown

Wayne N George

Jeffrey S Fisher

Arnaud Rival

Bala Murali Venkatesan

Tarun Kumar Khurana

Wenyi Feng

Jeffrey G Mandell

Yir-Shyuan Wu

David Louis Heiner

Chanfeng Zhao

Michal Lebl

Dajun Yuan

Jason Bryant

Lewis J Kraft

Mark J Nibbe

John A Moon

Boyan Boyanov

Craig M Ciesla

Dietrich A Dehlinger

Ali Agah

Alex Aravanis

Poorya Sabounchi

Bahram Ghaffarzadeh Kermani

Cyril Delattre

Arash Jamshidi

Aathavan Karunakaran

Yan-You Lin

Jorge Velarde

Michael S Graige

David L Barker

Marizel Rouilly

James M Tsay

David Reinhoudt

Xi-Jun Chen

Alex Alder

Vit Pokorny

Liangliang Qiang

Rohan N Akolkar

Jennifer Olivia Foley

Aaron Jones

Ren&eacute; Beerli

Todd Alan Dickinson

Hans Peter Herzig

Anthony W Czarnik

Alfredo E Bruno

Hamed Amini

Fabien Abeille

Shawn O Meade

Nels A Olson

Fiona E Black

Dirk Beckelmann

Kenny Chen

Nils Blom

Murali Venkatesan

Pargol Gheissari

Jennifer Wang

James Bierle

Markus Ehrat

Alfredo Emilio Bruno

Philippe Nussbaum

Reinhard Volkel

Majid Babazadeh

James Tsay

Shane M Bowen

A method for reducing sequencing by synthesis cycle time using a microfluidic device is provided. The microfluidic device comprises a flow cell having an inlet port, an outlet port, and a flow channel extending between the inlet port and the outlet port, wherein the flow channel receives an analyte of interest and one or more reagents for analyzing and detecting molecules. To aid in the acceleration of the reactions, the microfluidic device comprises a mixing device to increase the rates of diffusion of the reagents from the fluid bulk to an active surface of the flow cell. The mixing device comprises at least one of an electrothermal mixing device, an active mechanical mixing device, and a vibrational mixing device.

Methods and devices for mixing in a microfluidic system

Substrates comprising a functionalizable layer, a polymer layer comprising a plurality of micro-scale or nano-scale patterns, or combinations thereof, and a backing layer and the preparation thereof by using room-temperature UV nano-embossing processes are disclosed. The substrates can be prepared by a roll-to-roll continuous process. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.

Substrates comprising nano-patterning surfaces and methods of preparing thereof

A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

Compensator for multiple surface imaging

An example of a flow cell includes a substrate, which includes nano-depressions defined in a surface of the substrate, and interstitial regions separating the nano-depressions. A hydrophobic material layer has a surface that is at least substantially co-planar with the interstitial regions and is positioned to define a hydrophobic barrier around respective sub-sets of the nano-depressions.

Flow cells with a hydrophobic barrier

Example nanopore sequencers include a cis well, a trans well, and a nanopore fluidically connecting the cis and trans wells. In one example sequencer, a modified electrolyte (including an electrolyte and a cation complexing agent) is present in the cis well, or the trans well, or in the cis and the trans wells. In another example sequencer, a gel state polyelectrolyte is present in the cis well, or the trans well, or in the cis and the trans wells.

Nanopore sequencers

A labeled nucleotide includes a nucleotide, a linking molecule attached to a phosphate group of the nucleotide, and a redox-active charge tag attached to the linking molecule. The redox-active charge tag is to be oxidized or reduced by an electrically conductive channel when maintained in proximity of a sensing zone of the electrically conductive channel.

Labeled nucleotides

An example method includes contacting a substrate coated with a sol-gel material with a stamp that includes a plurality of protruding features. While contacting the coated sol-gel material with the stamp, the example method further includes curing the coated sol-gel material so as to form a patterned sol-gel layer that includes a plurality of wells. The stamp is separated from the patterned sol-gel layer.

Gel patterned surfaces

Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.

The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture.

Compounds and method for representational selection of nucleic acids from complex mixtures using hybridization

Compositions and methods for representational selection of nucleic acids from complex mixtures using hybridization

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San Diego, CA, United States of America

Steven Mark Barnard