Stemcell Technologies Inc.

Other

Notch Therapeutics (Canada), Inc.

Steven M Woodside

Oliver Egeler

Jason Everett Dowd

Terry Thomas

Andrew Mark Angus

Graeme Milton

Alessia Pallaoro

Richard Carpenedo

Gary Dossantos

Graeme Cross

Jason Dowd

The present disclosure provides a method for generating a T cell lineage population from progenitor T cells. The method comprises culturing the progenitor cells in the presence of Notch signalling ligand, such as Delta-like-4 (DL4), provided on a surface area of at least 7 square centimetres per millilitre culture volume (7 cm/mL). Population of cells produced using the method are provided as well as methods of using same. Further provided is a method of differentiating a progenitor T cell population enriched for CD4−CD8+ TCRγδ+ cells comprising culturing progenitor T cells in the presence of Notch signalling ligand, such as DL4, provided on a surface area of 0.78 to 4.7 cm/mL.

Method for generating T cell lineage populations from stem/progenitor cells

A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 90 degrees and less than 180 degrees.

Meniscus reducing member

A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 75 degrees and 110 degrees.

A magnetic particle comprises a polysaccharide matrix and a plurality of magnetic crystals dispersed in the matrix. A method for making magnetic particles comprises combining a basic solution with a metal ion solution and allowing the metal ions to oxidize to form magnetic crystals, and combining the magnetic crystals with a polysaccharide solution to form the magnetic particles.

Magnetic particles

An insert for a centrifuge tube suitable for use in density gradient separation is described. The insert includes a member sized to fit within the tube for dividing the tube into a top portion and a bottom portion. Optionally the insert has a support extending or depending from the member for positioning the member within the tube. At least two openings are located on the member so that a first opening is closer to a bottom end of the tube relative to a second opening when the insert is positioned in the centrifuge tube. Also described are methods for separating a target population of cells from a sample using the insert for a centrifuge tube.

Method and insert for density gradient separation

A cell culture device comprises a well. A plurality of microwells are within the well, and a first common fluid volume is within the well above the microwells. A set of sub-microwells are within each microwell, and a second common fluid volume is within each microwell above the set of sub-microwells.

Cell culture device

A meniscus reducing member for use in a vessel for containing a liquid including a surface feature overlying at least a portion of an interior surface of the vessel. The surface feature includes at least two surfaces for contacting the liquid that cooperate to reduce a width of a meniscus formed at an interface between the liquid and the surface feature by physically altering a contact angle between the liquid and the surface feature.

Cell culture vessels for meniscus reduction with aqueous solutions

The present application is directed to methods of improving cell culture vessel assays. In one aspect the application is directed to a method of reducing the curvature of the meniscus comprising applying a coating material to the interior wall of the vessel, wherein the coating material provides a receding contact angle of about 90 degrees with aqueous solutions and culture media. In another aspect, the application is directed to a method of labeling cells in a first solution by generating droplets of a second solution containing at least one cell-labelling agent and allowing the droplets of the second solution to contact the surface of the first solution.

Method of reducing curvature in a meniscus of liquid medium

A method of pipetting comprises (a) providing a pipette tip holder having a plurality of segregated compartments and a tip support member for supporting a plurality of pipette tips in the pipette tip holder, the tip support member configured to support a single pipette in tip each segregated compartment; (b providing a plurality of pipette tips in the tip support member; (c) removing a first pipette tip from a first segregated compartment; (d) using the first pipette tip to complete a pipetting operation and placing the first pipette tip into a segregated compartment that has not contained a used pipette tip; (e) subsequently removing the first pipette tip from the segregated compartment and using the first pipette tip to complete another pipetting operation; and, (f) replacing the first pipette tip into the pipette tip holder, whereby the first pipette tip, when placed in the segregated compartment, is segregated in the pipette tip holder to reduce the possibility of a fluid from the first pipette tip contacting other pipette tips in the pipette tip holder.

Method of pipetting using a pipette tip holder

The present invention is related to a method for separating a first population of cells from a second population of cells in a sample by discontinuous density gradient separation using dense particles to target the first population of cells and a density separation medium (DSM) that is at least about 0.001 g/cmhigher than the density of the second population of cells.

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Calgary, Canada

Steven M Woodside