Genetics Institute, Inc.

Wyeth

Steven M Vicik

Edward R LaVallie

Jeffrey S Deetz

James R Mercer

Neil L Schauer

Jonathan Lee Coffman

Jonathan L Coffman

Dwight Winters

Russell I Shpritzer

Jenifer L Thomas

Catherine A Briasco

Edward R La Vallie

Separation methods, for example, to isolate a recombinant protein, are disclosed. In some implementations, a method includes forming a solid containing a first cation and a first anion in a medium containing a protein, and separating the solid from the protein.

Separation methods

Methods are disclosed for refolding proteins of the TGF-&bgr; family of proteins. The methods employ as refolding agents one or more compounds which are acid substituted aminocyclohexanes.

Methods of refolding proteins

Methods are disclosed for refolding proteins of the TGF-&bgr; family of proteins. The methods employ as refolding agents one or more compounds which are non-detergent zwitterionic compounds, such as sulfobetaines, substituted pyridines, substituted pyrroles and acid substituted aminocyclohexanes.

Methods are disclosed for refolding proteins of the TGF-&bgr; family of proteins. The methods employ as refolding agents one or more compounds which are substituted pyridines.

Provided by the present invention are novel methods of protein recovery and purification methods and more specifically novel methods for the recovery and purification thioredoxin fusion proteins, especially of IL-11.

Growing community of inventors

Boxborough, MA, United States of America

Steven M Vicik