Litron Laboratories Ltd.

Other

Steven M Bryce

Stephen D Dertinger

Jeffrey C Bemis

The present invention relates a simple method for evaluating free eukaryotic cell nuclei for biomarkers of DNA damage and/or transcription factor activation, activity, or expression levels and/or epigenetic modifications to chromatin or chromatin-associated factors. The invention also teaches useful strategies for combining nuclear biomarkers into a matrix of endpoints that are capable of elucidating genotoxicants' primary mode of DNA-damaging activity. Kits for conducting methods according to the invention are also described.

Simplified nuclei analysis platform and biomarker matrix that supports genotoxic mode of action determinations

A vehicle driver notification assembly for notifying a driver when to drive forward includes a housing and a control circuit mounted in the housing. A sensor array is mounted on the housing and is electrically coupled to the control circuit. The sensor array detects parameters that indicate when a vehicle may proceed forward. The parameters include when a mass in front of a vehicle moves away from the vehicle and when the sensor array receives a proceed signal from a requisite traffic signaling device. An alert is electrically coupled to the control circuit and is turned on to provide an emission detectable by a person when the sensor array detects the parameters that allow the vehicle to proceed forward.

Vehicle driver notification assembly

The invention relates to methods and kits for the quantitative analysis of in vivo mutation frequencies of the Pig-A gene in individuals, particularly using peripheral blood samples of vertebrates.

Rapid in vivo gene mutation assay based on the Pig-A gene

The present invention relates a method for assessing in vivo hematotoxicity. The method utilizes differential staining of nucleated and non-nucleated blood cells, and also differential labeling of cells with functional versus dysfunctional mitochondrial membrane potential. Quantitative analyses can be conducted on stained whole blood specimens, and is based on blood cells' fluorescent emission and light scatter properties following exposure to an excitatory light source. The ratio of certain cell populations can be readily measured. Furthermore, it is also possible to express cell population values in terms of number per unit volume. This invention can be used to evaluate the hematotoxicity of drugs, chemicals, radiation, and other exogenous agents, or the effects that a suspected protective agent may have on induced hematotoxicity. Furthermore, the matrix of measurements provided by this invention is useful in estimating radiation dose, i.e., retrospectively. Kits for practicing the invention are also disclosed.

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Rochester, NY, United States of America

Steven M Bryce