Illumina Corporation

Steven M Bernard

John R Stuelpnagel

Andrew Antony Brown

Wayne N George

Maria Candelaria Rogert Bacigalupo

Jorge Velarde

Michael S Graige

David L Barker

James Tsay

Shane M Bowen

The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture.

Compositions and methods for representational selection of nucleic acids from complex mixtures using hybridization

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San Diego, CA, United States of America

Steven M Bernard