Dow Agrosciences LLC

Other

United States of America, as Represented by the Secretary of Agriculture

Corteva Agriscience LLC

Steven Lee Evans

Joseph F Petolino

Ryan C Blue

Jianquan Li

Kenneth Edwin Narva

H Ernest Schnepf

Steven Robert Webb

Lakshmi Sastry-Dent

David Richard Corbin

Manju Gupta

Donald J Merlo

Sandeep Kumar

James Wayne Bing

Ning Zhou

Joel Jay Sheets

Zehui Cao

W Michael Ainley

Kelley Ann Smith

Steven Arnold Thompson

Mary E Welter

Charles A Mihaliak

Geoffrey J Letchworth

This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.

Plant transactivation interaction motifs and uses thereof

The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

Precision gene targeting to a particular locus in maize

A universal donor polynucleotide is described that can be inserted at targeted locations in plant genomes to facilitate rapid and high throughput integration of a donor molecule within a specific genomic location.

Universal donor system for gene targeting

This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96-well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.

High-throughput DNA fragment assembly

Cysteine-specific radiolabeled Cry1Fa protein retains insecticidal activity against insect pests and binds to insect brush border membrane vesicle receptors in a saturable manner. The biologically-active radiolabeled Cry1Fa protein is useful in competitive binding assays with other Cry toxins.

Biologically-active radiolabeled Cry1Fa and receptor binding assay methods

This invention provides modified, insecticidal Cry35 proteins with enhanced properties as compared to wild-type Cry35 proteins. The modifications to these proteins were based in part on analysis of the atomic coordinates and three-dimensional (3D) structure of the &#x2dc;45 kDa 149B1 protein and other proteins in the Cry35 class. The subject invention also includes polynucleotides that encode these modified proteins, and transgenic plants that produce these modified proteins. This invention further provides methods of controlling plant pests, including rootworms, with these modified proteins. The modified proteins of the subject invention include chimeric toxins involving exchanged segments, domains, and motifs as discussed herein. The subject invention also provides methods of modifying Cry35 proteins.

Modified Cry35 proteins

The subject application provides various compositions of matter directed to West Nile virus (WNV) polypeptides and fragments thereof and polynucleotides, vectors and transformed host cells that encode, direct the expression of, or produce WNV polypeptides as set forth herein. Methods of using the polypeptides and polynucleotides for the production of immune responses in individuals or detecting the presence of WNV specific or neutralizing antibodies are also provided herein.

Plant-made west nile virus (WNV) vaccines, vectors and plant codon optimized sequences

Dumbbell shrug bench

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A vertically and angularly adjustable dumbbell holder is provided which allows for positioning of the dumbbell for various exercises. Multiple dumbbells may be supported from a single elongated weight bench and angularly and vertically positioned for a variety of exercises. The dumbbells are held in position by side supports when the dumbbell handles are used as a base support for exercises.

Weightbench dumbbell support apparatus

Elevated dumbbell holder

Growing community of inventors

Zionsville, IN, United States of America

Steven Lee Evans