Cargill Inc.

Novozymes A/s

Steven John Gort

Holly J Jessen

Olga V Selifonova

Ravi R Gokarn

Wolfgang Buckel

Thorsten Selmer

Hans H Liao

Paula M Hicks

Sara C McFarlan

Christopher Solheid

Toby Howard Richardson

David Paul Weiner

Peter Luginbuhl

Lishan Zhao

Ellen Burke

Fernando A Sanchez-Riera

Brian J Brazeau

Sherry R Kollmann

David B Weiner

Jose Miguel Laplaza

Mervyn L De Souza

Sara C Mcfarlan

Timothy Walter Abraham

Douglas Conrad Cameron, Jr

John P Rosazza

Mervyn Desouza

Mervyn L deSouza

Mary Jo Zidwick

Holly Jean Jessen

Sara C Mc Farlan

Sherry R Kollman

Jack Rosazza

This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof.

Aldolases, nucleic acids encoding them and methods for making and using them

Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed.

3-hydroxypropionic acid and other organic compounds

Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors

The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.

Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production

Methods and compositions that can be used to make monatin or salt thereof from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.

Production of monatin and monatin precursors

Methods of using beta-alanine/pyruvate aminotransferase to produce 3-hydroxypropionic acid and derivatives thereof, from beta-alanine, are disclosed. Cells and recombinant nucleic acids that can be used to practice the methods are also disclosed.

Production of 3-hydroxypropionic acid using beta-alanine/pyruvate aminotransferase

Growing community of inventors

Brooklyn Center, MN, United States of America

Steven John Gort