Sartec Corporation

Zirchrom Separations, Inc.

Steven J Rupp

Clayton V McNeff

Larry C McNeff

Peter W Carr

Dwight Stoll

Danielle R Hawker

Lorinda D Zigan

Kerry G Johnson

The present invention describes the use of porous metal oxides for the preparative purification of antibodies and biomolecules within a range of particle physical characteristics. Typically, particles from 15 to 100 microns in average diameter with pores sizes ranging from 400 to 600 angstroms and surface areas from 10 to 50 square meters per gram, and pore volumes from 0.1 to 0.4 mL/gram can be used for purification processes. The metal oxide particles that fall within this range of physical properties show enhanced utility and greater chromatographic capacity for antibodies than materials oxide particles falling outside of this range. Metal oxides such as zirconia, titania and alumina can all be modified with a multi-Lewis base moiety such as an organophosphate ethylenediamine-N,N-tetra(methylenephosphonic) acid (EDTPA), to produce a bio-compatible purification media for, biomolecules.

High stability porous metal oxide spherules used for one-step antibody purifications

A method and apparatus for controlling the addition of a liquid to a continuous flow of material. An algorithm is described for controlling the hydration process within a computerized grain delivery system. The grain delivery system operates by adding moisture to grain moving through the system, testing the moisture level of the hydrated grain and controlling the rate of flow of the wetting agent as a function of the wet grain moisture reading. In addition, a method is described for determining the relationship between dry grain moisture level, hydration level and the expected wet grain moisture level reading. Finally, a communications link is described which connects the grain delivery system to a remote computer.

Computerized grain delivery system

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Minnetonka, MN, United States of America

Steven J Rupp