Pioneer Hi-bred International, Inc.

Sakata Seed Corporation

Paladin Hybrids, Inc.

Steven F Fabijanski

Paul G Arnison

Laurian Sylvio Robert

Diego Albani

Johann Schernthaner

Tong Wu

Paul G Arinson

The present invention relates to novel methods for the transformation of crop species, novel methods for the selection and identification of transformed plant cells and novel methods for recovery of regenerated whole plants. The method also relates to the development of plants with novel traits and plants that contain novel recombinant DNA constructs.

Methods and constructs for plant transformation

A process is described for producing fertile hybrid seed or hybrid seed comprising fertile and sterile seed using male-sterile plants created by employing molecular techniques to manipulate anti-sense gene and other genes that are capable of controlling the production of fertile pollen in plants. Said plants are functionally male-sterile plants with pollen from male-fertile plants. Hybrid seed production is simplified and improved by this invention and can be extended to plant crop species for which commercially acceptable hybrid seed production methods are not currently available.

Molecular methods of hybrid seed production

A process is described for producing fertile hybrid seed or hybrid seed comprising fertile and sterile seed using male-sterile plants created by employing molecular techniques to manipulate anti-sense gene and other genes that are capable of controlling the production of fertile pollen in plants. These plants are functionally male-sterile plants with pollen from male-fertile plants. Hybrid seed production is simplified and improved by this invention and can be extended to plant crop species for which commercially acceptable hybrid seed production methods are not currently available.

A process is described for producing hybrid seed using male-sterile plants created by employing molecular techniques to manipulate antisense DNA and other genes that are capable of controlling the production of fertile pollen in plants. Transformation techniques are used to introduce constructs containing antisense DNA and other genes into plants. Said plants are functionally male-sterile and are useful for the production of hybrid seed by the crossing of said male-sterile plants with pollen from male-fertile plants. Hybrid seed production is simplified and improved by this invention and can be extended to plant crop species for which commercially acceptable hybrid seed production methods are not currently available.

Antisense gene systems of pollination control for hybrid seed production

A process is described for producing fertile hybrid seed or hybrid seed comprising fertile and sterile seed using male-sterile plants created by employing molecular techniques to manipulate genes that are capable of controlling the production of fertile pollen in plants. Hybrid seed production is simplified and improved by this approach, which can be extended to plant crop species for which commercially acceptable hybrid seed production methods have not been available.

The invention relates to a method for the preparation of a seed of a plant comprising crossing a male sterile plant and a second plant which is male fertile, and obtaining seed of said male sterile plant, said male sterile plant and said second plant being selected such that said seed has integrated into its genome a first recombinant DNA molecule having a first DNA sequence which encodes a first gene product and a first promoter which is capable of regulating the expression of said first DNA sequence, and a second recombinant DNA molecule which contains a second DNA sequence which encodes a second gene product and a second promoter which is capable of regulating the expression of said second DNA sequence, one of said first and said second recombinant DNA molecules originating from said male sterile plant and the other of said first and second recombinant molecules originating from said second plant, and said first and second gene products cooperating to selectively interfere with the function and/or development of cells of a plant that are critical to pollen formation and/or function of a plant grown from said seed whereby said plant grown from said seed is substantially male sterile. The invention also relates to a method of producing F1 hybrid seed from a plant regenerated from such seed, a method of producing F2 hybrid seed using plants regenerated from the F1 hybrid seed, the products produced from these methods and their use.

Growing community of inventors

Ottawa, Canada

Steven F Fabijanski