Bayer Corporation

Chiron Diagnostics Corporation

Steven E Bushnell

John E Monahan

Michael L Malek

Donna M Ford

Robert Schlegel

Howard J Kirchick

Wilson O Endege

Marcia E Lewis

Eddie Carroll, Iii

Jon H Astle

Theodore J Catino

Christopher C Burgess

Kathleen E Steinmann

Adnan Derti

Tina K Garyantes

This invention relates to novel human genes, to proteins expressed by the genes, and to variants of the proteins. The invention also relates to diagnostic assays and therapeutic agents related to the genes and proteins, including probes, antisense constructs. and antibodies. The subject nucleic acids have been found to be differentially regulated in tumor cells, particularly colon cancer cell lines and/or tissue.

Human genes and gene expression products

Method and apparatus for enabling resuspension wash and magnetic localization of sample components bound to particles with magnetic properties in reaction vessels during separation and wash for enhanced chemiluminescent signal generation in biomedical assays. The assays involve moving reaction vessels past magnetic arrays that partially localized the particles prior to passing a gap where washing occurs, with or without resuspension, after separating out the unbound components and liquid. The band of particles is further localized by a focusing magnet at the end of the array prior to dosing the vessel with acid for chemiluminescent purposes. A block of soft magnetic material is employed in place of a magnet in the gap to minimize magnetic strength at the gap. Trimmed magnets adjacent the gap cause left, then right, particle shifting that localizes the magnetizable particles. The gap enables improved resuspension by wash, whereas the localized particles enable more efficient resuspension by reagent.

Method and apparatus for wash, resuspension, recollection and

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Medfield, MA, United States of America

Steven E Bushnell