Coulter Corporation

Steven E Bott

Frank J Lucas

Gerald E Jaffe

James H Carter

William H Hart

Brian D Schmitz

An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.

Method for determining activity of enzymes in metabolically active whole

A method for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound is also disclosed.

A fiber optic spatial filter assembly for laser diffraction particle sizing apparatus utilizing a laser to generate a monochromatic light beam which is coupled to an optical fiber operating substantially in a monomode and creating a beam of light having a high degree of spatial coherence which is then passed through collimating lenses to interrogate and impinge upon the particles of matter through which the laser diffracted light passes. The light scattered by the particles is focused onto a Fourier plane and thereafter impinges upon a photooptical detector array, positioned coincident with the Fourier plane, for measuring the light intensities of the scattered light by scattering angle, thus enabling the computation of particle size.

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Pembroke Pines, FL, United States of America

Steven E Bott