Butamax Advanced Biofuels LLC

Danisco US Inc.

Steven D Doig

Hongxian He

Chris Leeflang

Ranjan Patnaik

Ryan L Frisch

Marc Anton Bernhard Kolkman

Frank Wouter Koopman

Ritu Bhalla

Kakasaheb Suresh Konde

Douglas Robert Anton

Bruce Aaron Diner

The present disclosure is generally related to compositions and methods for obtainingcells/strains having increased protein production capabilities. Certain embodiments of the disclosure are related to genetically modifiedcells/strains derived from parentalcells/strains comprising a variant rghR2 gene.

Compositions and methods for increased protein production in

Compositions and methods for increased protein production in bacillus licheniformis

Provided herein are processes for producing an improved culture of cells comprising an engineered butanol biosynthetic pathway. The processes comprise (a) providing a cell culture of recombinant microorganisms comprising an engineered butanol biosynthetic pathway, wherein the engineered butanol biosynthetic pathway is minimal or not activated; and (b) growing the culture of recombinant microorganisms under adaptive conditions whereby pathway activation is increased to produce an improved cell culture and whereby the improved cell culture is capable of continuing to grow in fermentation.

Partial adaption for butanol production

Partial adaptation for butanol production

Provided herein are processes comprising providing a fermentation medium comprising a fermentable carbon source, a recombinant microorganism comprising an engineered butanol biosynthetic pathway, and butanol; contacting said fermentation medium with an extractant composition comprising an effective amount of antioxidant or antioxidant-like compound, whereby at least a portion of the butanol in the fermentation medium partitions into the extractant; recovering at least a portion of the butanol and extractant composition from the fermentation medium; recycling the extractant composition recovered in (c) one or more times in the fermentation medium; optionally adjusting the effective amount of antioxidant or antioxidant-like compound in the extractant composition, whereby the rate of oxidation or effect of oxidized products in the recycled extractant composition is substantially reduced and/or avoided such that the extractant composition may be recycled.

Recyclable extractant compositions

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Wilmington, DE, United States of America

Steven D Doig