Butamax Advanced Biofuels LLC

Nutrition & Biosciences USA 4, Inc.

E.i. Dupont De Nemours and Company

Butamax (tm) Advanced Biofuels LLC

Inbiose N.v.

Steven Cary Rothman

Yougen Li

Jessica McElvain

Lori Ann Maggio-Hall

Larry Cameron Anthony

Mark Scott Payne

Rick Weizhang Ye

Hongxian He

Robert DiCosimo

Qiong Cheng

Daniel P O'Keefe

Mark J Nelson

Arthur Leo Kruckeberg

Jean-Francois Tomb

Brian James Paul

Ranjan Patnaik

Zheng You

Lixuan Lisa Huang

Kristin Ruebling-Jass

Daniel P Okeefe

Arthur Ouwehand

Jian Ping Lai

Jeremy S Minshull

Sridhar Govindarajan

Dennis Flint

Der-Ing Liao

Wonchul Suh

Lisa L Huang

Alexander Vincent Tobias

Kerry Hollands

Lori Anne Maggio-hall

Jessica Mcelvain

Katharine J Gibson

Daniel P O'keefe

An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.

Enzymatic synthesis of soluble glucan fiber

Microbial cells genetically engineered with a heterologous nucleic acid sequence that increases export of 2′ fucosyllactose are disclosed. Methods of increasing export of 2′ fucosyllactose from a microbial cell and for identifying a heterologous nucleic acid sequence that increases export of 2′ fucosyllactose from a microbial cell are also disclosed.

Increasing export of 2'fucosyllactose from microbial cells through the expression of a heterologous nucleic acid

An enzymatically produced soluble &#x3b1;-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble &#x3b1;-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble &#x3b1;-glucan fiber are also provided.

Polypeptides having ketol-acid reductoisomerase activity are provided. Also disclosed are recombinant host cells comprising isobutanol biosynthetic pathways employing such polypeptides. Methods for producing isobutanol employing host cells comprising the polypeptides having ketol-acid reductoisomerase activity are also disclosed.

Polypeptides with ketol-acid reductoisomerase activity

Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.

DHAD variants for butanol production

Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.

Host cells and methods for production of isobutanol

Provided herein are polypeptides having ketol-acid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.

Ketol-acid reductoisomerase enzymes and methods of use

Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.

Keto-isovalerate decarboxylase enzymes and methods of use thereof

Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe&#x2014;S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe&#x2014;S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe&#x2014;S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.

Recombinant yeast host cells comprising FE-S cluster proteins

Provided herein are polypeptides having ketol-aid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.

Growing community of inventors

Wilmington, DE, United States of America

Steven Cary Rothman