Caliper Technologies Corporation

Caliper Life Sciences, Inc.

Other

Affymax Technologies, N.v.

Canon U.S. Life Sciences, Inc.

Intel Corporation

Canon U.s.a., Inc.

Affymetrix, Inc.

Applera Corporation

Steven A Sundberg

J Wallace Parce

Calvin Y H Chow

Andrea W Chow

Hua Yang

Anne R Kopf-Sill

Michael R Knapp

Edward P Donlon

Jeffrey A Wolk

Wesley Bryan Dong

Aaron J Rulison

Ivor T Knight

Deborah John Boles

Robert John Moti

Michael Slater

Andrew Fabans

Allen R Boronkay

Christopher P Holmes

Robert S Dubrow

Ronald W Barrett

Lubert Stryer

Michael C Pirrung

Calvin Y Chow

Joseph E Kercso

Andrew W Toth

Xing Su

John Wallace Parce

Grace M Credo

David Fujimoto

Claudia B Cohen

Glenn H McGall

Luc J Bousse

Jeffrey William Jacobs

Ring-Ling Chien

H Garrett Wada

Tammy Burd Mehta

Steven James Gallagher

Benjamin N Wang

John Robert Schullek

Edward Timofeey

Andrei Mirzabekov

Walter Ausserer

Claudia L Poglitsch

Deborah J Boles

Edward Dolon

Allen Borankay

The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

Method and apparatus for generating thermal melting curves in a microfluidic device

Embodiments of the invention provide devices and methods for extracting nucleic acid molecules from solution using electric fields. The structures and methods of embodiments of the invention are suited to incorporation into micro and nano fluidic devices, such as lab-on-a-chip devices and micro total analysis systems.

Nanofabricated structures for electric field-assisted nucleic acid extraction

Fluid introduction is facilitated through the use of a port which extends entirely through a microfluidic substrate. Capillary forces can be used to retain the fluid within the port, and a series of samples or other fluids may be introduced through a single port by sequentially blowing the fluid out through the substrate and replacing the removed fluid with an alternate fluid, or by displacing the fluid in part with additional fluid. In another aspect, microfluidic substrates have channels which varying in cross-sectional dimension so that capillary action spreads a fluid only within a limited portion of the channel network. In yet another aspect, the introduction ports may include a multiplicity of very small channels leading from the port to a fluid channel, so as to filter out particles or other contaminants which might otherwise block the channel at the junction between the channel and the introduction port.

Microfabricated structures for facilitating fluid introduction into microfluidic devices

The invention provides improved systems, devices, and methods for analyzing a large number of sample compounds contained in standard multi-well microtiter plates or other array structures. The multi-well plates travel along a conveyor system to a test station having a microfluidic device. At the test station, each plate is removed from the conveyor and the wells of the multi-well plate are sequentially aligned with an input port of the microfluidic device. After at least a portion of each sample has been input into the microfluidic channel system, the plate is returned to the conveyor system. Pre and/or post testing stations may be disposed along the conveyor system, and the use of an X-Y-Z robotic arm and novel plate support bracket allows each of the samples in the wells to be input into the microfluidic network through a probe affixed to a microfluidic chip. A clamshell structure having a hinged lid can releasably support the chip while providing and/or accommodating the electrical, optical, structural, and other interface connections between the microfluidic device and the surrounding system.

High throughput microfluidic systems and methods

Growing community of inventors

San Francisco, CA, United States of America

Steven A Sundberg