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Steven A Seiden

Marc Irwin Epstein

Louis W Seiden

Micah I Krichevsky

Sam Butz

This invention fills several voids in bioreactor technology that allows efficient connection of aspects of physical science (optics, electronics, physical chemistry, sensors) to aspects of microbial and cell culture physiology in a uniquely interactive manner. This is accomplished mathematically through decision making software that utilizes detected changes in the course of fermentation. Decisions are aimed at determining the optima for cellular growth, optimizing for production or degradation of metabolites or substrates, or determining the limits of growth under various combinations of conditions. The invention determines optima or limits in a manner more quickly and at less cost than traditional methods. The basis for the computer generated decisions may be first or second derivative changes observed such as inflection points, limits on allowable rates of change, or the like. The most common measured parameter controlling the decision making process is the optically observed growth of the cells (e.g. microbial, animal, or plant cell cultures) under study. Any other measurable parameter (e.g. pH, temperature, pigment production) may be used to control the process (i.e., the independent variable). This process and variations of this process on a laboratory scale are valuable for research and development, education, pilot plant models, and bio-manufacturing optimization, including scale up to production volumes.

Decision-making spectral bioreactor

The invention relates to devices and methods for ultrasonically degassing a sealed container of fluid such as a container of beer, wine or soda. The invention further relates to methods and apparatus for measuring the respiration rate of bacteria in a test liquid containing bacteria such as waste water or activated sludge. The invention also relates to methods for measuring one or more components of the gas released from the sealed container utilizing ultrasonic and other degassing techniques and to methods for expanding head space of the container.

Degassing techniques applied to sealed containers for beverages, waste

This invention is directed to a device which measures the oxygen component of a beverage gas using a specific oxygen probe, ultrasonic degassing, a special valving technique, and microprocessor based software. The measurement is made in the gaseous state in a two chamber system. The device is controlled by an electronic console that is built around a microprocessor which sequences and times the valves, receives the data from the oxygen probe and its accompanying temperature compensation circuit, and displays the data. An alternative method is to use several chambers and one pass. Additional chambers may be used to increase the speed of the test, control interferences, or aid in identifying gases other than the oxygen component. The device may also have an interface piercing head manifold that allows CO.sub.2 and oxygen to be tested in the same container and in one preparation. The invention also relates to specific gas measurements with non-specific type measurements and the general techniques can be applied to environmental problems that involve oxygen demand and respiration of bacteria.

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Raleigh, NC, United States of America

Steven A Seiden