Ibis Biosciences, Inc.

Isis Pharmaceuticals, Inc.

Steven A Hofstadler

Richard Holmes Griffey

David J Ecker

Rangarajan Sampath

John McNeil

Jared James Drader

Stanley T Crooke

Thomas A Hall

Lawrence B Blyn

Rex O Bare

James C Hannis

Jose R Gutierrez

Jeffrey Christopher Smith

Paul J Gleason

Robert D Miller

Mark W Eshoo

Eric E Swayze

Yun Jiang

Jared H Nathanson

Vivek Samant

Kristin Sannes Lowery

Amy L Schink

Gordon Bruce Collier

Kevin Oberkramer

Lendell Cummins

Ronald K Bergold

Neill K White

Raymond Ranken

Kristin Sannes-Lowery

Venkatraman Mohan

Vanessa Harpin

Christian Massire

Sherilynn Manalili Wheeler

Ray Ranken

Susan M Freier

C Frank Bennett

Brenda F Baker

Andrew J Scherer

Roland Stoughton

Lendell L Cummins

Ranga Sampath

Stanley T Crook

Dino J Sofianos

Karl H Rudnick

Duane J Knize

Cecil L Basham, Jr

Clifford T Lewis

Brons M Larson

Jonathan R Bar-on

Dennis P Murray

David W Robbins

John P Penhune

Neil White

Kevin S Oberkramer

The present invention compositions, methods and systems to identify, detect, and/or quantify bacterial DNA in the presence of contaminating non-bacterial DNA. In particular, the present invention provides oligonucleotides configured to detect a relatively small amount of bacterial DNA in the presence of an overwhelmingly large amount of contaminating human DNA.

Bioagent detection oligonucleotides

Provided herein is technology relating to testing biological samples and particularly, but not exclusively, to devices, systems, and kits for performing multiple, simultaneous real-time assays on a sample in a single-use disposable format. For example, the technology relates to an apparatus that finds use, for example, for point-of-care diagnostics, including use at accident sites, emergency rooms, in surgery, in intensive care units, as well as for non-medical applications.

Multiple-analyte assay device and system

The present invention realtes to systems and methods for amplifying nucleic acid. In particular, systems and methods are provided for inhibiting polymerase based terminal transferase activity within a polynucleotide amplification setting (e.g., polymerase chain reaction). In addition, systems and methods are provided for generating amplified products generated with polynucleotide amplification techniques having reduced 3' non-templated nucleotide addition.

Compositions and methods for inhibiting terminal transferase activity

The present invention provides methods for rapid forensic analysis of mitochondrial DNA and methods for characterizing heteroplasmy of mitochondrial DNA, which can be used to assess the progression of mitochondrial diseases.

Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy

The present invention provides methods of: identifying pathogens in biological samples from humans and animals, resolving a plurality of etiologic agents present in samples obtained from humans and animals, determining detailed genetic information about such pathogens or etiologic agents, and rapid detection and identification of bioagents from environmental, clinical or other samples.

Methods for rapid identification of pathogens in humans and animals

The present invention relates the use of broad range primer (e.g., as broad range capture olignucleotides) immobilized in a SCODA method gel to allow, for example, selective concentration of target nucleic acids. Such concentrated target nucleic acids may, for example, be: i) eluted from the gel and analyzed (e.g., by broad range primer methods); ii) subject to in situ (e.g., in gel) PCR methods; and/or iii) analyzed in the gel (e.g., by fluorescent detection methods).

Bioagent detection methods

The methods described herein generally relate to characterization of large analytes, such as biomolecules, by molecular mass analysis. Specifically, the methods are directed to molecular mass analysis of singly- or multiply-charged ions by selective ion filtering carried out by a digital thresholding process.

Mass spectrometry with selective ion filtration by digital thresholding

The present invention provides methods for the quantification of an unknown bioagent in a sample by amplification of nucleic acid of the bioagent, and concurrent amplification of a known quantity of a calibration polynucleotide from which are obtained a bioagent identifying amplicon and a calibration amplicon. Upon molecular mass analysis, mass and abundance data are obtained. The identity of the bioagent is then determined from the molecular mass of the bioagent identifying amplicon and the quantity of the identified bioagent in the sample is determined from the abundance data of the bioagent identifying amplicon and the abundance data of the calibration amplicon.

Methods for concurrent identification and quantification of an unknown bioagent

The present invention relates to portable systems and devices, and corresponding methods, for detecting bioagents. In particular, the present invention provides systems, devices, and methods that utilize one or more of a sample preparation component, sample analysis component employing broad range primers, and sample detection component.

Bioagent detection systems, devices, and methods

The present invention provides systems and methods for analysis of samples, particularly biological and environmental sample to detect biomolecules of interest contained therein. A variety of system components are described herein, including, but not limited to, components for sample handling, mixing of materials, sample processing, transfer of materials, and analysis of materials. The invention further provides mechanisms for combining and integrating the different components and for housing, moving, and storing system components or the system as a whole. The systems may include any one or more or all of these components. The system finds particular use when employed for analysis of nucleic acid molecule using mass spectrometry, however, the invention is not limited such specific uses.

Systems for bioagent indentification

The invention relates generally to sample ionization, and provides ionization probe assemblies, systems, computer program products, and methods useful for this purpose.

Ionization probe assemblies

Provided herein is technology relating to testing biological samples and particularly, but not exclusively, to methods for performing multiple, simultaneous real-time assays on a sample in a single-use disposable format. For example, the technology relates to methods employing an apparatus that finds use, for example, for point-of-care diagnostics, including use at accident sites, emergency rooms, in surgery, in intensive care units, as well as for non-medical applications.

The present invention relates to systems and methods for amplifying nucleic acid. In particular, systems and methods are provided for inhibiting polymerase based terminal transferase activity within a polynucleotide amplification setting (e.g., polymerase chain reaction). In addition, systems and methods are provided for generating amplified products generated with polynucleotide amplification techniques having reduced 3' non-templated nucleotide addition.

Growing community of inventors

Vista, CA, United States of America

Steven A Hofstadler