Perkinelmer Singapore Pte Limited

Steve Morris Bush

Robert Alan Hoult

Patrick Courtney

Alistair Fitch

Shab Ladha

Colin Blackmore

George Robert Sealy

Dionisis Korontzis

An assembly () and a method are provided for inputting a light beam into a light path extending from the confocal scanning head () to the microscope () of a scanning confocal microscope system to illuminate a selected region of a sample mounted in the microscope. The assembly comprises a light input for receiving a light beam from a light source; beam directing means () for controlling the path of the light beam with reference to the shape of the selected region of the sample; and a beam coupler () for selective coupling the light beam into the light path from the confocal scanning head () to the microscope (), with the beam direction being controlled by the beam directing means so as to illuminate the selected region. A scanning confocal microscope system including such an assembly is also described, together with a method of calibrating the system. In addition, an optical switch for selectively switching a light beam between two paths is disclosed.

Relating to scanning confocal microscopy

A method of analysing a sample of biological material including components (to) tagged with respective fluorescent labels (to), comprises the steps of identifying a specific labeled component () to be monitored, and irradiating with an energy beam at least one region () of the sample which substantially surrounds said specific component. The energy beam modifies the optical properties of labels in the region () such that they are distinguishable from the label () associated with said specific component (). This assists the tracking of a specific component of interest as it moves through a sample. Apparatus for carrying out the method is also described.

Method and apparatus for analyzing a dynamic sample

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Wycombe, United Kingdom

Steve Morris Bush