Dade Behring Marburg Gmbh

Siemens Healthcare Diagnostics Products Gmbh

Steve De Keczer

Edwin F Ullman

Dariush Davalian

Nurith Kurn

Rajendra Singh

John S Pease

Remy Cromer

Yen Ping Liu

Rajesh Patel

Methods are disclosed for determining minute quantities of an analyte in a medium suspected of containing the analyte. One method comprises treating a medium suspected of containing an analyte under conditions such that the analyte, if present, causes a substrate having an oxidant cleavable linker and a photosensitizer to come into close proximity. The photosensitizer generates singlet oxygen which oxidatively cleaves the linker to form a product which can be detected in a sandwich detection assay such as LOCI. The amount of product detected is related to the amount of analyte in the medium. Compositions, kits, and compounds are also disclosed.

Amplified signal in binding assays

Alpha-haloketones are useful alkylating agents for coupling to sulfhydryl-containing biomolecules. However, they react spontaneously with water, alkali and organic bases and therefore cannot be stored for extended periods of time in aqueous solutions, particularly in the presence of proteins at physiological pH. The present invention provides novel solutions to these problems, however, as novel compounds and compositions comprising protected haloketones are disclosed herein. Methods of preparing and using protected haloketones which are useful in a variety of applications&mdash;e.g., in assays and conjugation reactions&mdash;are also disclosed herein.

Protecting groups for biological labeling

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Saratoga, CA, United States of America

Steve De Keczer