Ajinomoto Co., Ltd.

Stephen Van Dien

Yoshihiro Usuda

Kazuhiko Matsui

Shintaro Iwatani

Takuji Ueda

Yuichiro Tsuji

Tomoko Suzuki

Mika Moriya

Yuta Nakai

A bacterium belonging to the genuswhich has an ability to produce L-lysine or L-threonine and which is modified so that a malic enzyme does not function normally in a cell, and a method for producing L-lysine or L-threonine, comprising culturing the bacterium in a medium to produce and cause accumulation of L-lysine or L-threonine, and collecting the L-lysine or L-threonine from the medium.

Method for producing L-lysine or L-threonine

A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.

Method for determining metabolic flux affecting substance production

A method for determining metabolic flux distribution of a cell, which comprises the steps of: 

Method for determining metabolic flux

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Kawasaki, Japan

Stephen Van Dien