Novartis Finance Corporation

Ciba-geigy Corporation

Other

The United States of America As Represented by the United States

Stephen T Lam

Dwight Steven Hill

Thomas D Gaffney

Philip E Hammer

James M Ligon

John A Ryals

James Madison Ligon

Scott Joseph Uknes

Thomas Schupp

James Joseph Beck

Jeffrey I Stein

Karl-Heinz Van Pee

Sabine Kirner

Nancy Torkewitz

Thomas R Young

Charles R Howell

Daniel Murray Ellis

Nancy R Torkewitz

Patrice Dion

Chandra S Nautiyal

J Ole Becker

The present invention is directed to the production of pyrrolnitrin in a host via recombinant expression of the polypeptides needed to biologically synthesize pyrrolnitrin. Genes isolated from P. fluorescens, P. pyrrocinia, B. cepacia, and M. fulvus that encode polypeptides necessary to produce pyrrolnitrin are provided, along with methods for identifying and isolating genes needed to recombinantly biosynthesize pyrrolnitrin from any organism capable of producing pyrrolnitrin. The isolated genes may be transformed and expressed in a desired host organisms to produce pyrrolnitrin according to the invention for a variety of purposes, including protecting the host from a pathogen, developing the host as a biocontrol agent, and producing large, uniform amounts of pyrrolnitrin.

Pyrrolnitrin biosynthesis genes and uses thereof

The present invention exploits the discovery that certain single base changes in the gacA gene coding sequence, which result in certain single amino acid changes in the encoded GacA protein, render it LemA-independent. The present invention therefore provides DNA that encodes mutant GacA proteins that do not require phosphorylation by LemA in order to be active as transcriptional activators.

DNA encoding lemA-independent GacA and its use in activating gene

The present invention is directed to the production of an antipathogenic substance (APS) in a host via recombinant expression of the polypeptides needed to biologically synthesize the APS. Genes encoding polypeptides necessary to produce particular antipathogenic substances are provided, along with methods for identifying and isolating genes needed to recombinantly biosynthesize any desired APS. The cloned genes may be transformed and expressed in a desired host organisms to produce the APS according to the invention for a variety of purposes, including protecting the host from a pathogen, developing the host as a biocontrol agent, and producing large, uniform amounts of the APS.

Genes for the synthesis of pyrrolnitrin

Strains of Pseudomonas have been genetically engineered to have enhanced biocontrol properties. The strains of the invention are particularly effective against plant pathogenic fungi such as species of Rhizoctonia and Pythium, because the strains produce enhanced amounts of antifungal metabolites such as pyrrolnitrin that are active against these fungal pathogens. Both the genetically modified biocontrol strains and the antifungal metabolites can be used as active agents for biocontrol compositions.

Genetically modified Pseudomonas strains with enhanced biocontrol

Pyrrolnitrin biosynthesis genes

Gene activating sequences which activate the expression of other bacterial genes, which are latent or expressed at low levels, are provided. The gene activating sequences confer the ability to produce several metabolites and may be transferred to bacterial strains. The transformed biocontrol agents are active to inhibit the growth of the fungal pathogens.

Genomic DNA encoding a Pseudomonas global transcriptional activation

Method of protecting plants by transformation with genes for the

DNA encoding the pseudomonas fluorescens sensor/kinase element, lemA,

Genomic DNA encoding the pseudomonas fluorescens alternative sigma

Genes for the synthesis of antipathogenic substances

Genomic DNA encoding a pseudomonas global transcriptional activation

Genes for the synthesis of antipathogenic substance

The present invention relates to a method for enhancing colonization of desired microorganisms in a localized environment comprising introducing into said localized environment a genetically engineered microorganism comprising a gene comprising a DNA sequence coding for one or more substrate utilizing protein, wherein said substrate utilizing protein confers a competitive effective ability to utilize a substrate by a microorganism in which it is genetically engineered, as well as the isolated genes and the genetically engineered microorganisms of such a method.

Microorganisms with mannopine catabolizing ability

Growing community of inventors

Raleigh, NC, United States of America

Stephen T Lam