Biomerieux Sa

Biomerieux, Inc.

Stephen Stanforth

Arthur James

Sylvain Orenga

John Perry

Vindhya Salwatura

Olivier Fabrega

Annette Rigby

Vindhya Lakshika Salwatura

The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W, W, Wand Ware independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination of same; n=0, 1 or 2; X is NR, CZZ, S or O, R being H, alkyl, aralkyl, aryl, alkanoic or alkylsulphonic, Zand Zbeing an alkyl; Y is N or NR, R being alkyl, aralkyl, aryl, alkanoic or alkylsulphonic; Z, Z, Zand Zare independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulphonyl, including the sulphonyl or carboxyl amides or esters thereof, and the salts of same. 

Nitroreductase enzymatic substrates

The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity, wherein: W, W, Wand Ware independently H, Br, Cl, F, I, alkyl, alkoxy, thiomethyl, perfluoroalkyl, nitro, cyano, carboxyl (including the esters or amides thereof) or any combination of same; n=0, 1 or 2; U is N or NR and V is CZN or NR or V is N or NR and U is CZ, R being H, alkyl, aralkyl, aryl, alkanoic or alkylsulphonic; and Z, Z, Z, Z, Zand Zare independently H, Br, Cl, F, I, alkyl, aryl, alkoxy, perfluoroalkyl, nitro, cyano, carboxyl, sulphonyl, including the sulphonyl or carboxyl amides or esters thereof, and the salts of same.

The present invention relates to the use of a compound of the following formula (I), as an enzyme substrate for the detection of a peptidase activity or as a pH indicator: according to which:

Peptidase substrates

The invention relates to the use of a compound having formula (I) as an enzymatic substrate for the detection of a nitroreductase activity or as an enzymatic substrate for the detection of a nitroreductase activity and indicator of pH, wherein: 

The present invention relates to the use of a compound of the following formula (I) for detecting a peptidase activity and/or a variation in pH: according to which:

The present invention relates to the use of a compound of the following formula (I) for detecting a peptidase activity and/or a variation in pH: 

An enzyme substrate of formula (I) detects peptidase activity: Methods of detecting peptidase activity of a microorganism may include incubating a biological sample with the enzyme substrate of formula (I), and detecting peptidase activity if a microorganism having peptidase activity is present in the biological sample. The enzyme substrate may be included in a reaction medium.

Substrates for detecting peptidase activity and methods using the substrates

The present invention relates to an enzyme substrate for detecting nitroreductase activity of formula (I) below: in which X is S, NX1, O or NX1-CO; R1 is nothing or a substituent selected from Cl, CH, Br, F, I, alkyl, aryl and carboxyl; R2 is nothing or a substituent selected from Cl, O&#x2014;CH&#x2014;O, O&#x2014;CH, F, diethylenediamine-CH, NR3R4, Br, I, alkyl, aryl, carboxyl, NOand R3 and R4 are independently H or an alkyl group containing from 1 to 4 carbon atoms; and X1 is selected from H, CH, CHPh, OH, alkyl and aryl.

The present invention relates to methods for the detection, identification and/or quantification of microorganisms with peptidase activity using enzymatic substrates with the following general formula: 

Method for the detection, identification and/or quantification of microoganisms with peptidase activity using phenoxazinone derivatives as enzyme substrates

The present invention relates to novel enzymatic substrates with the following general formula: where R, R, R, R, R, R, A and X are as defined in claim 1, the reaction media comprising the same and the use thereof for the detection and/or identification and/or quantification of microorganisms expressing at least one peptidase activity.

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Northumberland, United Kingdom

Stephen Stanforth