USA As Represented by Secretary of the Navy

Naval Medical Research Center

University of Colorado

Stephen Savarino

Patricia Guerry

Mario Artur Monteiro

Michael G Jobling

Randall K Holmes

The inventive subject matter relates to a construct comprising antigens derived from multiple enterobacteria includingcapsule polysaccharide polymer, enterotoxigenicrecombinant polypeptide construct and lipopolysaccharide fromspp. The subject invention also relates to a method of inducing an immune response utilizing the inventive composition.

Combined enteropathogen recombinant construct

The inventive subject matter relates to a recombinant polypeptide constructs comprising enterotoxigenicfimbrial subunits. The recombinant polypeptide constructs comprise multiple subunits to the same or different ETEC fimbrial types. The constructs are useful for inclusion in immunogenic formulations for the induction of immunity against entertoxigenic. The inventive subject matter also relates to the use of the recombinant polypeptide constructs in induce anti-enterotoxigenicimmunity.

Recombinant polypeptide construct comprising multiple enterotoxigenicfimbrial subunits

The inventive subject matter relates to the methods for the induction of immunity and prevention of diarrhea resulting from. The inventive subject matter also relates to the useadhesins as immunogens and to the construction of conformationally stability and protease resistantadhesin constructs useful for inducing immunity topathogenic bacteria. The methods provide for the induction of B-cell mediated immunity and for the induction of antibody capable of inhibiting the adherence and colonization of, including enterotoxigenic, to human cells.

Adhesin as immunogen against enterotoxigenic

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The inventive subject matter relates to a recombinant polypeptide construct comprising enterotoxigenicfimbrial subunits. The recombinant polypeptide constructs comprise multiple subunits to the same or different ETEC fimbrial types. The constructs are useful for inclusion in immunogenic formulations for the inductin of immunity against entertoxigenic. The inventive subject matter also relates to the use of the recombinant polypeptide constructs in induce anti-enterotoxigenic

The inventive subject matter relates to an immunogenic composition composed of a chimeric molecule including a conformationally stable adhesin fromfused to a bacterial toxin A subunit, such as cholera toxin A subunit or heat-labiletoxin A subunit. The invention also relates to the adhesin-toxin chimera noncovalently associated with a toxin B subunit of the same or different species as the A subunit. The invention also relates to a method of utilizing an adhesin/toxin fusion composition to elicit an immune response.

The invention relates to an immunogenic composition and method of the immunogenic composition for the production and administration of a passive immunoprophylactic against enterotoxigenic. The immunoprophylactic is made collecting anti-adhesin in the colostrum or milk of vaccinated domesticated animals such as cows. The immunoprophylactic is administered either as a dietary supplement or in capsular or tablet form.

Anti-adhesin based passive immunoprophylactic

The inventive subject matter relates to the methods for the induction of immunity and prevention of diarrhea resulting from. The inventive subject matter also relates to the useadhesins as immunogens and to the construction of conformationally stability and protease resistantadhesin constructs useful for inducing immunity topathogenic bacteria. The methods provide for the induction of B-cell mediated immunity and for the induction of antibody capable of inhibiting the adherence and colonization ofincluding enterotoxigenic, to human cells.

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Kensington, MD, United States of America

Stephen Savarino