Leland Stanford Junior University

Stephen S Hamann

Olav Solgaard

Joseph Russell Landry

An axially swept light sheet fluorescence microscope has illumination optics capable scanning the focus region of a line beam along an illumination optical axis to illuminate a light sheet in a sample plane, and detection optics capable of collecting fluorescence light from the sample plane and imaging the collected light on a light detector with a rolling shutter. A microcontroller synchronizes the rolling shutter with the scanning of the focus region. The illumination optics performs the axial scanning using a linear phased array of independently controllable electrostatically driven optical elements controlled by the microcontroller.

Rapid axial scanning for light sheet microscopy using a phased array

A patterned line of optical radiation can be steered in the other two directions (e.g., line patterned in y, steered in x and z) with a 1-D phase shifter array in a Fourier optics configuration. Preferably the patterned line is provided by forming a line focus and modulating it with an array of grating light valve devices in an amplitude modulation configuration. Phase modulation is preferably provided with an array of grating light valve devices in a phase modulation configuration.

Growing community of inventors

Mountain View, CA, United States of America

Stephen S Hamann