Sanofi

The Salk Institute for Biological Studies

Stephen Rush Fuhs

Jacques Mauger

Tony Hunter

Fahad A Al-Obeidi

Magda Stankova

Jill Meisenhelder

Robert Alan Binnie

Robert A Binne

A peptide is disclosed of the general structure: Z&#x2014;W&#x2014;Y, wherein Z and Y are independently a one to eight amino acid sequence wherein the amino acids are selected from glycine and alanine and W is a non-hydrolyzable pHis analogue. Such peptides can be used to produce sequence-independent anti-phosphohistidine antibodies. Also provided are antibodies that specifically bind to a peptide comprising a phosphohistidine (or a non-hydrolyzable pHis analogue) but fail to specifically bind to an identical peptide containing histidine instead of phosphohistidine.

Compositions and methods for analyzing histidine phosphorylation

Isolated monoclonal antibodies and or antigen binding fragments thereof are disclosed herein that specifically bind polypeptides comprising a histidine phosphorylated at N3 (3-pHis). Nucleic acids encoding these antibodies, vectors including these nucleic acids, and host cells transformed with these vectors and nucleic acids are also disclosed. Methods are also disclosed for using these antibodies, such as for detection of polypeptides comprising a histidine phosphorylated at N3 (3-pHis). In some embodiments, the methods can be used to investigate signal transduction pathways.

Generation and use of polyclonal and monoclonal antibodies specific for 3-phosphohistidine

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San Diego, CA, United States of America

Stephen Rush Fuhs