Henkel Corporation

Henkel Research Corporation

Stephen Picataggio

L Dudley Eirich

Tracy Rohrer

James M Cregg

Martin Anthony Gleeson

Lisa Haas

Kristine Deanda

The omega-hydroxylase activity in the yeast Candida tropicalis is increased by amplification of the P450ALK and P450RED genes. The specific productivity (grams of dicarboxylic acid/liter/hr) of substantially pure alpha, omega-dicarboxylic acids is increased by culturing C. tropicalis strains having amplified P450ALK and P450RED genes.

Method for increasing the omega-hydroxylase activity in candida tropicals

Method for increasing the omega-hydroxylase activity in Candida

The POX genes of C. tropicalis are disrupted resulting in the complete blockage of the beta-oxidation pathway in the strain. Fermentation of C. tropicalis cells having disrupted genes on alkane, fatty acid and fatty acid ester substrates produces substantially pure dicarboxylic acids in substantially quantitative yield.

Site-specific modification of the candida tropicals genome

A transformation system is provided for C. tropicalis, comprising constructs and microorganisms, as well as methods for preparing constructs and microorganisms, and transforming microorganisms. Particularly, a yeast transformation system comprising auxotrophic hosts which are auxotrophic in either an amino acid, purine or pyrimidine pathways and employ DNA constructs comprising genes encoding biosynthetic enzymes which functionally complement the auxotrophies to prototrophies.

Candida tropicalis transformation system

Growing community of inventors

Santa Rosa, CA, United States of America

Stephen Picataggio