The Scripps Research Institute

University of California

Sapphire Energy, Inc.

Triton Algae Innovations, Inc.

Stephen P Mayfield

Michael Mendez

Yan Poon

Bryan O'Neill

Miller Tran

Beth Ann Rasala

Xun Wang

Michael Mayfield

Kari Mikkelson

Jon Hansen

Scott Franklin

Oscar Gonzalez

Robert McBride

Austin Hallgren

David Ryan Georgianna

Melissa Scranton

James A Gregory

Joseph Ostrand

Anthony Jason Erich Berndt

John Tzu-Yu Chang

Carla S Jones

Michael J Hannon

This invention provides synthetic promoters capable of promoting and/or initiating transcription of a polynucleotide in an algal cell, and methods of designing, producing and using such promoters.

Synthetic algal promoters

Provided herein are methods for the production of high density cultures of algae as well as nutritional supplements from such cultures and the use of such cultures to produce therapeutic proteins.

Method for growing algae

Provided herein are methods for the treatment of GI tract diseases and conditions and/or symptoms associated with such diseases and conditions in a mammal. Also provided are preparations and compositions for use in the methods. Also provided are methods of growing and preparingbiomass for administration to a mammal in need of treatment of GI tract diseases and conditions and/or symptoms associated with such diseases and conditions.

Method of treating alimentary canal conditions

Provided are colostrum and milk polypeptides recombinantly expressed in photosynthetic organisms containing colostrum and milk polypeptides, compositions comprising such organisms and methods for producing such organisms.

Colostrum/milk protein compositions

Provided are chloroplasts engineered to recombinantly express mammalian colostrum and milk polypeptides, photosynthetic organisms containing such chloroplasts, and compositions comprising such organisms and methods for producing such organisms. In certain embodiments, provided is a chloroplast comprising one or more polynucleotides encoding one or more mammalian milk or colostrum polypeptides selected from osteopontin, lactadherin, cathelicidin-1, lysozyme, lactoperoxidase, lingual antimicrobial peptide (LAP), alpha-lactalbumin, and soluble CD14.

Chloroplast expressing colostrum or milk polypeptides

The present invention relates to the production of malaria transmission blocking vaccines in single-celled green algae, particularly algae of the genus, e.g.,; the immunogenicpolypeptides produced and compositions comprising them; and methods for preventing, ameliorating, reducing, delaying, treating and blocking the transmission of malaria by administration of immunogenicpolypeptides produced in an algal host cell.

Algal produced malarial transmission blocking vaccines

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.

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The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.

High throughput screening of genetically modified photosynthetic organisms

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

Expression of eukaryotic polypeptides in chloroplasts

Use of genetically modified organisms to generate biomass degrading enzymes

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery or proteins/peptides, especially gut active proteins, without purification is disclosed.

Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast

RNA binding protein and binding site useful for expression of recombinant molecules

The present invention relates to recombinant protein disulfide isomerase, RB60, that functions as a translational regulator of the binding of a translational activator protein, RB47, to its binding site for the activation of translation. The recombinant RB60 protein is useful in a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants.

Growing community of inventors

Cardiff, CA, United States of America

Stephen P Mayfield