Life Technologies Corporation

Nautilus Subsidiary, Inc.

Other

Nautilus Biotechnology, Inc.

Lift Technologies Corporation

Stephen P Hendricks

Marian Peris

Michael Phelan

Parag Mallick

Lei Xi

Torri Elise Rinker

Tural Aksel

Elvis Ikwa

Pierre F Indermuhle

David G King

Sadie Ingle

Steven Tan

Barnett B Rosenblum

Cheng-Yao Chen

Daniel Mazur

Xinzhan Peng

Christina Inman

Amy Castillo

Keith P Bjornson

Dmitriy Gremyachinskiy

David Ruff

Zhaochun Ma

Shiaw-Min Chen

Michael Dorwart

Paul M Kenney

Elana Swartzman

Mark Shannon

Dennis E Prosen

Gregory Kapp

Rachel Galimidi

Sonal Tonapi

Joshua Simon Klein

Julia Robinson

Cassandra Stawicki

Julia Lu

Roland Nagel

Markus Burns

Christina E Inman

Pengyu Hao

Lei (Larry) Xi

David J King

Compositions, systems, and methods for the display of analytes such as biomolecules are described. Display of analytes is achieved by coupling of the analytes to displaying molecules that are configured to associate with surfaces or interfaces. Arrays of analytes may be formed from the described systems for utilization in assays and other methods.

Systems and methods for biomolecule retention

Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein.

Polymerase compositions and methods

An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.

Affinity reagents having enhanced binding and detection characteristics

Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.

Enzymatic ligation of nucleic acids

Apparatus and method for converting a gas-powered vehicle to an electric vehicle. For example, one embodiment of an apparatus comprises: a motor case comprising: an inner surface forming a central cavity with a volume sufficient to enclose a first type of electric motor, the volume of the central cavity formed, at least in part, based on dimensions of a crankshaft of a gas-powered engine to be replaced by an electric motor; an outer surface coupled to the inner surface through a material layer comprising a material from which the motor case is fabricated; a plurality of internal connection elements to fixedly couple the motor within the motor case; and a set of external connection elements coupled to or formed on the outer surface of the motor case, the set of external connection elements arranged in accordance with connection points of a vehicle chassis, the set of external connection elements to be used to couple the motor case to the chassis at the connection points and to couple various gas-powered engine accessories. In one implementation, a second set of external connection elements are used to connect accessories, including accessories which are in combination with the electric motor and accessories which are not used, but which are coupled to the motor case for aesthetic purposes.

Method and apparatus for conversion of motorized vehicles to electric vehicles

This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

Workflow for detection of ligands using nucleic acids

This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.

Polymerase assay with a FRET substrate

In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5' phosphate group by removing an adenylate group from a terminal 5&#x2032; phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5&#x2032; phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Enhanced ligation reactions

Growing community of inventors

Los Gatos, CA, United States of America

Stephen P Hendricks