Old Dominion University Research Foundation

Stephen P Beebe

Karl H Schoenbach

Richard M Heller

Shu Xiao

James Thomas Camp

Amy Donate

Juergen Friedrich Kolb

Systems and methods for treatment of a biological tissues including target tissues and other tissues. The method includes elevating a temperature of the target tissues above a physiological temperature of the biological tissues to treatment temperature, and generating an electric field extending through at least a portion of the target tissues using a pre-defined sequence of short voltage pulses applied between at least two electrodes. In the method, the treatment temperature is maintained during the generating. Further, the pre-defined sequence is selected such that a magnitude of the electric field generated is sufficient to induce electromanipulation in the portion of the target tissues without substantially elevating of the temperature of the portion of the target tissues above the treatment temperature.

Electromanipulation of cells and other biological tissues by pulsed electric fields at elevated temperatures

Systems and methods for treatment of a biological tissues comprising target tissues and other tissues. The method includes elevating a temperature of the target tissues above a physiological temperature of the biological tissues to treatment temperature, and generating an electric field extending through at least a portion of the target tissues using a pre-defined sequence of short voltage pulses applied between at least two electrodes. In the method, the treatment temperature is maintained during the generating. Further, the pre-defined sequence is selected such that a magnitude of the electric field generated is sufficient to induce electromanipulation in the portion of the target tissues without substantially elevating of the temperature of the portion of the target tissues above the treatment temperature.

An instrumentfor delivering a high voltage pulse to tissue is disclosed. The instrumentcan include an outer support memberwith a liquid reservoirthat has a liquid-contacting interior surface, an openingat a distal endof the outer support member, and a ground electrodeextending in a longitudinal direction and having a lower surfaceproximate the opening. The instrumentcan also include a working electrodeextending longitudinally from the liquid-contacting interior surfacewith a needle-shaped distal portionproximate the distal end; and an inlet portand an outlet portin liquid communication with the liquid reservoir. The working electrodecan be electrically isolated from the ground electrodeby an insulating portionof the outer support member, and a direct path can exist through the liquid reservoirbetween the ground electrodeand the working electrode

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Norfolk, VA, United States of America

Stephen P Beebe