Dow Agrosciences LLC

Ms-technologies Gmbh

Sangamo Biosciences, Inc.

Sangamo Therapeutics, Inc.

Corteva Agriscience LLC

Stephen N Novak

Ning Zhou

Yunxing Cory Cui

Manju Gupta

Sean Michael Russell

Terry R Wright

Bruce Marvin Held

Thomas Hoffman

Sandra Grace Toledo

Vaithilingam Sekar

Dawn M Parkhurst

Julissa Colon

Sandeep Kumar

Jeffrey C Miller

Fyodor Urnov

Joseph F Petolino

Russell DeKelver

Andrew F Worden

Ryan M Lee

Asha Mehra Palta

Sunita Gopalan

Thomas W Greene

Asha M Palta

David Richard Corbin

German Carlos Spangenberg

Steven Robert Webb

Jyoti R Rout

Zehui Cao

Matthew J Henry

Wei Chen

Tina Kaiser

John White Forster

Andrew Asberry

Noel Cogan

Matthew Hayden

Tim Sawbridge

Liu Shen

John Mason

Sivarama Reddy Chennareddy

W Mike Ainley

Dawn Parkhurst

Sandra Toledo

Yunxing Cui

Bruce Held

Sean Russell

Terry Wright

Thomas William Greene

Liu Yin Shen

Thom Hoffman

Russell Dekelver

John Forster

This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be 'stacked' with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TAQMAN® PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof

This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be 'stacked' with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

Disclosed herein are methods and compositions for the repair of site specific nuclease binding sites by targeted integration and/or targeted excision of one or more sequences into a cell.

Reconstruction of site specific nuclease binding sites

The current disclosure relates to methods and compositions for improving plant varieties through plant breeding and plant genetics. For instance, the disclosure concerns increasing the recombination frequency of a heterozygous trait genetically linked to a second trait within plants. Further, the disclosure concerns breaking the genetic linkage between a first allele and a second allele.

Methodologies and compositions for creating targeted recombination and breaking linkage between traits

An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5' and 3&#x2032; ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Engineered transgene integration platform (ETIP) for gene targeting and trait stacking

The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

Engineered zinc finger proteins targeting 5-enolpyruvyl shikimate-3-phosphate synthase genes

The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.

Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis

Disclosed herein are methods for determining if a contaminating integration of a nucleotide sequence is present in a set of nucleic acids. Further disclosed herein are methods for determining the copy number/zygosity of a nucleic acid sequence of interest. The methods disclosed herein may be performed using quantitative PCR.

Triplex event-specific reaction used to quantify specific events and possible contaminating events

This invention relates to soybean event pDAB8291.45.36.2, which includes a novel expression cassette comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding, and herbicide tolerant plants. In some embodiments, the event sequence can be 'stacked' with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to detection methods, including endpoint TaqMan PCR assays, for the detection of Event pDAB8291.45.36.2 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

Stacked herbicide tolerance event 8291.45.36.2, related transgenic soybean lines, and detection thereof

Growing community of inventors

Westfield, IN, United States of America

Stephen N Novak