E.i. Dupont De Nemours and Company

Midwest Research Institute

Synthetic Genomics, Inc.

Genencor International, Inc.

Stephen K Picataggio

Quinn Qun Zhu

Narendra S Yadav

Hongxiang Zhang

Howard Glenn Damude

Zhixiong Xue

Daniel Joseph Macool

James John Ragghianti

Mark Scott Payne

Donald Eugene Trimbur

Min Zhang

Ramesh V Nair

Peter John Gillies

Philippe Soucaille

Dana M Walters Pollak

John E Seip

Mark Finkelstein

Qiong Cheng

Fernando Valle

Pierre E Rouviere

James M Odom

Kevin T Watts

Mattheos Koffas

Kevin V Martin

Amy Kuang-Hsu

Kristine A Deanda

Christina K Eddy

Rick Weizhang Ye

Jean-Francois Tomb

Robert Christopher Brown

Kelley C Norton

Gena Roy

Jessica Roxane Kristof

Tao Wang

Andreas Schenzle

Prachee Prakash

Stuart A Underwood

Hao Ni

Deana J Dicosimo

Vasantha Nagarajan

Gregory Marshall Whited

Sharon Loretta Haynie

Charles Edwin Nakamura

Paul Gordon Roessler

Yat-Chen Chou

Mary Ann Franden

John Verruto

Anthony Arthur Gatenby

Maria R Diaz-Torres

Patricia C Brzostowicz

Ali Mohagheghi

Amy Kuang-Hua Hsu

Mildred M Newman

Amy K Hsu

Fernado Valle

Richard D La Reau

James D Mc Millan

James D McMillan

Deana DiCosimo

Edward S Miller

The present invention relates to methods for the production of &#x3c9;-3 and/or &#x3c9;-6 fatty acids in oleaginous yeast. Thus, desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to &#x3b3;-linolenic acid (GLA); &#x3b1;-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-&#x3b3;-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to arachidonic acid (ARA); ETA to eicosapentaenoic acid (EPA); DGLA to ETA; EPA to docosapentaenoic acid (DPA); and ARA to EPA have been introduced into the genome offor synthesis of ARA and EPA.

Production of polyunsaturated fatty acids in oleaginous yeasts

This invention describes genes, metabolic pathways, microbial strains and methods to produce methylbutanol and other compounds of interest from renewable feedstocks.

Methylbutanol as an advanced biofuel

Engineered strains of the oleaginous yeastcapable of producing greater than 25% eicosapentaenoic acid (EPA, an &#x3c9;-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.

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Methods to increase the percent of polyunsaturated fatty acids (PUFAs) within the total lipids and oils of PUFA-producing oleaginous organisms are provided herein, by regulating the activity of specific acyltransferases. Specifically, since oil biosynthesis is expected to compete with polyunsaturation during oleaginy, it is possible to reduce or inactivate the activity of an organism's DAG ATs (e.g., phospholipid:diacylglycerol acyltransferase (PDAT) and/or diacylglycerol acyltransferase 1 (DGAT1) and/or diacylglycerol acyltransferase 2 (DGAT2)) to thereby reduce the overall rate of oil biosynthesis while concomitantly increasing the percent of PUFAs that are incorporated into the lipid and oil fractions. The teachings herein will thereby enable one to engineer a wide variety of oleaginous organisms to produce oils with very specific fatty acid compositions.

Acyltransferase regulation to increase the percent of polyunsaturated fatty acids in total lipids and oils of oleaginous organisms

An engineered strain of the oleaginous yeastcapable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.

Docosahexaenoic acid producing strains of yarrowia lipolytica

This invention describes genes, metabolic pathways, microbial strains and methods to produce 2,6-dimethyloctane as an advanced biofuel from renewable feedstocks.

Dimethyloctane as an advanced biofuel

This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

Promoter and plasmid system for genetic engineering

This invention describes genes, metabolic pathways, microbial strains and methods to produce methyl butanol and other compounds of interest from renewable feedstocks.

The present invention relates to fatty acid desaturases and elongases able to catalyze the conversion of linoleic acid (LA) to &#x3b3;-linolenic acid (GLA); &#x3b1;-linoleic acid (ALA) to stearidonic acid (STA); GLA to dihomo-&#x3b3;-linoleic acid (DGLA); STA to eicosatetraenoic acid (ETA); DGLA to ETA; eicosapentaenoic acid (EPA) to docosapentaenoic acid (DPA); and arachidonic acid (ARA) to EPA. Nucleic acid sequences encoding codon-optimized desaturases and elongases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the codon-optimized desaturase or elongases, and recombinant host microorganisms expressing increased levels of desaturase or elongase are described.

Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts

Engineered strains of the oleaginous yeastcapable of producing greater than 10% arachidonic acid (ARA, an &#x3c9;-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.

Growing community of inventors

Landenberg, PA, United States of America

Stephen K Picataggio