General Electric Company

Stephen James Capper

John Richard Nelson

Ryan Charles Heller

Alison Myfanwy Wakefield

Paresh Lakhubhai Patel

Peter James Tatnell

Jeffrey Kenneth Horton

Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3' end and a 5&#x2032; end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (T) of the primer at least 10&#xb0; C. below the reaction temperature. The amplification is effected under isothermal condition.

Isothermal amplification under low salt condition

Solid phase isothermal amplification

Growing community of inventors

Cardiff, United Kingdom

Stephen James Capper