Becton Dickinson and Company Limited

Arbor Vita Corporation

Other

Millipore Corporation

Stephen J Lovell

Stephen C Wardlaw

Robert A Levine

Peter S Lu

Rodolfo R Rodriguez

Leon W Terstappen

Adrien P Malick

John D Mantlo

Robert W Rosenstein

Johannes Schweizer

Alvydas J Ozinskas

Virginia M Crews

Kristen L Manion

Nancy Hasse

Lydia Blank

Subhash Dhanesar

Song Shi

Thomas M Gentle

John Patrick Carr

Richard Ivey

Alan J Weiss

John K Shuler

John Gamel

Jeffrey H Bruton

Abigail S Fisher

Richard T Root

Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent, neutralizing the pH of the intermediate composition to produce the protein extract. Kits and compositions for practicing the subject methods are also provided.

Method of protein extraction from cells

Methods for diagnosis of HPV infection in a subject are provided. HPV infection in a subject can be determined by generating mass profile data for a biological sample from the subject and correlating the mass profile data with reference mass profiles to detect the presence or absence, and/or quantity of at least one biomarker associated with HPV infection. Methods for detecting at least one biomarker associated with HPV infection in a biological sample are also provided.

Methods for diagnosing oncogenic human papillomavirus (HPV)

Disclosed is multi-segmented fabricated gear rim having two distinct steel layers, an inner layer, and an outer layer from which gear teeth are cut. The inner and outer layers are molecularly bonded to each other, with the inner layer being a high weldability layer and the outer layer being a high hardenability layer.

Bimetallic gear rim

The present invention relates to a device for determining an analyte in a fluid sample in an assay. The device comprises a lid having a plurality of elements and a membrane. The elements have openings which allow passage of fluid to the membrane. The membrane is supported by the lid and covers the openings of the elements. The undersurface of the membrane is in contact with a porous wick which contains a first zone which includes binder attached to a particulate label and a second zone containing salt. The wick extends downward from the membrane and is in contact with the fluid sample.

Device for determining an analyte in a sample

A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. Each density-defined body carries analyte-capture binding materials such as antigens or antibodies, which are specific to an epitope, or other specific high affinity binding site on a target analyte which target analyte may be in the blood or other sample being tested; and the level of which analyte is indicative of the patient's health. At least one labeled binding material which is also specific to an epitope, or other specific high affinity binding site on the target analyte is added to the sample so as to form labeled binding material/analyte/body complexes in the sample. Upon centrifugation, the complexes will settle out in different areas in the tube according to the respective density of the body or bodies; and the degree of label emission of the complex layers can enable qualitative and/or quantitative analyses of the sample to be made. Unbound labeled binding materials will be separated from the complexed layers by the washing action of ascending or descending components of the sample during the centrifugation step. Unbound labeled binding material will thus not interfere with the analysis.

Assay of blood or other biologic samples for target analytes

The present invention relates to a method and assay device for detecting small analytes. The results of the assay can be directly read from the device, which is a lateral flow device.

Direct read lateral flow assay for small analytes

An indirect assay for analyte employs a particle derivatized with a plurality of molecules of one or more compounds to increase assay sensitivity. In an indirect sandwich assay format, at least one of the compounds is a binder for the analyte, and the tracer is comprised of a binder for at least one of the compounds on the particle. In this manner, a plurality of tracer molecules may be indirectly bound to a single analyte molecule which is bound in a complex formed in the assay.

Amplified assay for analyte

Growing community of inventors

Lutherville, MD, United States of America

Stephen J Lovell