G.d. Searle & Company

Glaxo Laboratories Limited

Stephen J Brewer

Ian D Fleming

Michael K Turner

Helmut M Sassenfeld

Fused-Polypeptides comprising a polypeptide product and a covalently bonded charged amino acid polymer are disclosed. Methods of detecting the presence of the charged amino acid polymer are used to quantitate the presence of the fused-polypeptides either in crude or purified solutions. The charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide product. Examples using polyarginine and carboxypeptidase B to produce urogastrone and gamma interferon are included.

Fused polypeptides and methods for their detection

A method for the production of polypeptides utilizing a polymer of charged amino acids and an exopeptidase. A DNA sequence coding for the charged amino acid polymer is inserted adjacent to one end of the polypeptide gene. The polypeptide-charged amino acid polymer fusion product is removed from most contaminants by selecting for the charged amino acid polymer during a first isolation step. Next the charged amino acid polymer is hydrolyzed using an exopeptidase which leaves the polypeptide active. A second isolation step removes the polypeptide from any remaining contaminents. An example using polyarginine and carboxypeptidase B to produce urogastrone is included.

Process for the preparation of polypeptides utilizing a charged amino

A novel O-transcarbamoylase enzyme and its use in preparing a variety of 3-carbamoyloxymethyl cephalosporins from 3-hydroxymethyl cephalosporins and carbamoyl compounds are described.

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High Wycombe, United Kingdom

Stephen J Brewer