University of Kansas

Other

Stephen H Benedict

Marcia A Chan

Scott A Tibbetts

Teruna J Siahaan

Kelli M Cool

Teruna J Siaiiann

Abby L Dotson

Iwao Nozawa

A method for generating a purified regulatory T cell composition having a CD4CD25Foxp3phenotype from naive T cells having CD4CD45RAROCD11aCD27phenotype comprising the steps of stimulating a T cell receptor on the naive T cells and stimulating ICAM-1 on the naive T cells.

Preparation of regulatory T cells using ICAM-1 co-stimulation

Peptide compositions which inhibit the binding of one protein to another protein, and corresponding methods of use are disclosed. These peptide compositions include at least one peptide which binds to one protein, and at least one peptide which binds to the other protein. In the preferred embodiment, the peptide composition is composed of a combination of cyclic ICAM-1-based and LFA-1-based peptides which inhibit the binding of LFA-1 to ICAM-1. Such LFA-1/ICAM-1-based peptide compositions can be used to treat disease states such as rejection of transplanted organs, allergies, and autoimmune diseases.

Peptide Compositions which induce immune tolerance and methods of use

Methods and compositions useful in inhibiting integrin-associated diseases are provided. The compositions contain a polypeptide having one or more D-amino acids that inhibit integrin binding to its associated binding polypeptide. The compositions include retro-inverso polypeptides.

D-form polypeptide that inhibits the interaction between LFA-1 and ICAM-1

Peptide compositions which induce immune tolerance and methods of use

Short-chain peptides replicating fragments of functional domains derived from LFA-1 and ICAM-1 parent protein sequences serve to modulate the ICAM/LFA binding interaction. In one aspect of the invention, this modulation serves to block interprotein binding reactivity, as a peptide of the invention binds to a target protein in a manner that precludes the normal binding reaction between ICAM-1 and LFA-1. In another aspect of the invention, this modulation enhances the reactivity of a first peptide, as a second peptide induces a conformational change in the target protein from a first conformation to a second, more reactive, conformation. The peptides are used according to a method including the steps of providing the proteins and applying them to a population of cells.

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Lawrence, KS, United States of America

Stephen H Benedict