Roche Molecular Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Stephen Gordon Will

Amar Gupta

Veeraiah Bodepudi

Nicolas Newton

Janet Kornegay

Carrie L Aldrich

Thomas W Myers

David H Gelfand

Ellen H Fiss

Alison Tsan

Fred Lawrence Reichert

Nancy J Schoenbrunner

Dirk Zimmermann

Meike Eickhoff

Niclas Hitziger

Joachim Glaubitz

Andreas Trapp

Rachel Shahinian

Christopher D Newhouse

Siegfried Dick

Alexander Schmelzer

Markus Fischer

Dieter Heindl

Jenny A Johnson

Roy A Bohenzky

Edward Soh Smith

Hannes Kuchelmeister

Kalyani Mangipudi

Igor Kozlov

Yuker Wang

John Niemiec

Alexander Nierth

Heike Wilts

Lori Steiner

Lin Wu

Concordio Anacleto

Christian Wellner

Janine Montiel

Laura Geyer

Anke Mussmann

Amar P Gupta

Rachel Langland

Christopher Newhouse

Roy Bohenzky

A multi-layer plasma separation card comprising (a) a first layer including a sample receiving member comprising (i) a top planar surface for applying or receiving a blood sample, said sample receiving portion being adapted to permit contact of said blood sample with a separating member; and (ii) a bottom planar surface being adapted to contact said separating member, (b) a second layer including at least three separating members, each separating member being adapted to permit the passage of plasma to an absorptive member and comprising (i) a top planar surface for receiving said blood sample; and (ii) a bottom planar shield-shaped surface being adapted to contact said absorptive member, and (c) a third layer including at least two absorptive members for absorbing plasma from the bottom planar surface of each corresponding separating member and a backing member arranged in a manner to support said absorptive members, each absorptive member comprising a removable absorptive element having a top planar surface being adapted to contact said bottom planar surface of the separating member, said absorptive element is detachable fixed to the third layer.

Plasma separation card

The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside triphosphates. The present invention also provides for methods for using the modified nucleoside triphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.

Photocleavable nucleotide reagents with high stability

Provided herein are methods and compositions to effectively isolate nucleic acids using protein-based sample collection matrices.

Protein-based sample collection matrices and devices

Methods and kits for joining fragmented nucleic acid sequences together are provided, including performing an amplifying step including contacting a sample suspected of including a fragmented target nucleic acid with a pair of external primers and a pair of self-complementary internal primers, and generating a full length target nucleic acid. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, kits are provided that are designed for the detection of a target nucleic acid sequence.

Methods and kits for joining fragmented nucleic acids together

A method is provided for allele-specific amplification, utilizing a blocking oligonucleotide including at least one nucleotide with a base covalently modified at the exocyclic amino group, the blocking oligonucleotide being perfectly complementary to a wild type (WT) sequence when hybridized forming a first complex having a first melting temperature (Tm), the blocking oligonucleotide being partially non-complementary, at one or more nucleotides, to a target mutant (MT) sequence when hybridized forming a second complex having a second melting temperature (Tm), wherein the first Tm is higher than the second Tm and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the blocking oligonucleotide becomes unhybridized from the target MT sequence during amplification but remains hybridized with the WT sequence inhibiting amplification of the WT sequence utilizing a polymerase lacking 5'-3&#x2032; nuclease activity.

Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression

Photoblocked probes are disclosed including a specific hydrolysis probe having a first nucleic acid sequence complementary to a target having a second nucleic acid sequence, a first and a second interactive labels, a 5' end and a 3&#x2032; end, and one or more photocleavable moieties coupled to one or more nucleotides of the specific hydrolysis probe, wherein the photocleaveable moiety interferes with the hybridization of the specific hydrolysis probe with the region of the amplification product. Also disclosed are PCR methods for the detection of the presence or absence of a target nucleic acid in a sample utilizing the photoblocked probes, as well as kits.

Photoblocked probes and methods for sequential detection of nucleic acids

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having a 3'-terminal nucleotide complementary to only one variant of the target sequence and having at least one nucleotide with a base covalently modified at the exocyclic amino group, wherein the allele-specific oligonucleotide is extended by a nucleotide-incorporating biocatalyst predominantly when hybridized to the variant of the target sequence for which it has said complementary 3&#x2032;-terminal nucleotide.

Allele-specific amplification

The present invention provides reagents for use in the amplification of nucleic acids. Amplification carried out using oligonucleotides containing modified nucleotides can result in less non-specific amplification compared to amplification carried out using unmodified oligonucleotides.

Methods and reagents for reducing non-specific amplification

Alkyl amines act to release formaldehyde cross-linking that occurs in biological samples. Thus, contacting alkyl amines to formaldehyde fixed samples is a useful way to render biological components of the samples, including nucleic acids or proteins, more accessible to detection and characterization.

Alkyl amines improve detection of components of formaldehyde-fixed biological samples

A method for isolating a biological target material from a biological sample is provided, the method including binding the target material to a solid support, wherein impurities are selectively removed from the solid support by washing the latter with a wash buffer containing a cationic amine. A kit for isolating a biological target material from a biological sample is also provided, the kit including a wash buffer containing a cationic amine, and the use of a respective wash buffer.

Amine compounds for the selective preparation of biological samples

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, having an internally-placed selective nucleotide complementary to only one variant of the target sequence wherein the allele-specific oligonucleotide is extended by a nucleic acid polymerase predominantly or exclusively when hybridized to the variant of the target sequence for which it has said complementary selective nucleotide.

Allele-specific amplification of nucleic acids

Methods are provided for the amplification of at least a first and a second target nucleic acid that may be present in at least one fluid sample using an internal control nucleic acid for qualitative and/or quantitative purposes.

Control nucleic acids for multiple parameters

The present invention provides a method for the amplification of at least a first and a second target nucleic acid that may be present in a fluid sample. The invention further provides a kit and an analytical system for carrying out said amplification.

Generic PCR

The present invention provides methods and reagents for use in the amplification of nucleic acids. Amplification carried out using oligonucleotides containing modified nucleotides can result in less non-specific amplification compared to amplification carried out using unmodified oligonucleotides.

Growing community of inventors

Oakland, CA, United States of America

Stephen Gordon Will