Inscripta, Inc.

Stephen Federowicz

Michael Graige

Deanna Church

Andrew Garst

Aamir Mir

Kyle Seamon

The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

Methods for identifying selective binding pairs

The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

Automated instrumentation for production of T-cell receptor peptide libraries

The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

Cascade/dCas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells

Automated instrumentation for production of peptide libraries

The present disclosure automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptide antigens to identify potential TCR binding targets. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

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Boulder, CO, United States of America

Stephen Federowicz