Coulter Corporation

Stephen F Healy, Jr

Robert H Raynor

Olavi Siiman

John A Maples

Melissa J Stiglitz

Kenneth H Kortright

Martin L Golick

Michael L Rice

Preparation of non-infectious control cells from normal, non-infectious, non-disease altered blood by depletion or augmentation of one or more cell types found in normal blood to reflect a specific disease state. The control cells so produced are preserved and thereafter reconstituted for use in immunological assays.

Preparation of preserved, non-infectious control cell for use in the

The invention describes the preparation of non-infectious control cells from normal, non-infectious, non-disease altered blood by depletion or augmentation of one or more cell types found in normal blood to reflect a specific disease state. The control cells so produced are preserved and thereafter reconstituted for use in immunological assays.

Preserved, non-infectious control cell for use in the identification of

A method of lyophilizing mammalian cells to produce preserved human cells, hybridoma cell lines, tissue cells, and control cells for immunoassays and other hematological measurements. Prior to freezing and lyophilizing, the prepared mammalian cell pellet is suspended in a solution of trehalose in an isotonic fluid prepared at a specified optimal concentration and incubated at room temperature for a designated time period. The lyophilized cells when rehydrated, retain their optimal physiological characteristics suitable for use as an analytical control and retain said characteristics after storage at 2.degree.-8.degree. C. for a period in excess of five (5) months.

Stabilized lyophilized mammalian cells and method of making same

A control slide for use in an immunoassay having a section of a cell pellet retained on the slide in a stable and substantially permanent formation which can be stored at room temperature. Further, the invention includes the method of making the control slide.

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Miami, FL, United States of America

Stephen F Healy, Jr