Laboratory Corporation of America Holdings

Stephen Erickson

Jose S Gil

Dwight Lyman Anderson

Minh Mindy Bao Nguyen

Ben Barrett Hopkins

Wendy S Hahn

Andrew J Conrad

Ekaterina Kovacheva

John Paulson

Jessica Stach

Matthew J Brown

Dwight L Anderson

Wendy Hahn

Disclosed herein are compositions, methods, kits and systems for rapid detection of microorganisms using a reproduction-deficient indicator bacteriophage. The specificity of such reproduction-deficient indicator bacteriophage for binding and infecting particular microorganisms of interest allows targeted and sensitive detection of a microorganism of interest.

Devices and methods for detecting microorganisms using recombinant reproduction-deficient indicator bacteriophage

Disclosed herein are methods and systems for rapid detection of microorganisms such asspp. in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as-specific bacteriophage, allows detection of a specific microorganism, such asspp. and an indicator signal may be amplified to optimize assay sensitivity.

Methods and systems for the rapid detection of cronobacter using infectious agents

Disclosed herein are methods and systems for rapid detection of microorganisms in a sample, without culturing for enrichment of the microorganism. A modified bacteriophage is also disclosed which comprises a non-native indicator gene in the late gene region. The indicator product is not a fusion protein. The specificity of infectious agents allows a specific microorganism to be targeted, and an indicator signal may be amplified to optimize assay sensitivity.

Methods and systems for rapid detection of microorganisms using infectious agents

Disclosed herein are methods and systems for rapid detection of antibiotic resistance of a microorganism in a sample. A modified recombinant phage is also disclosed which comprises an indicator gene in the late gene region. The specificity of infectious agents allows a specific microorganism to be targeted, and an indicator signal may be amplified to optimize assay sensitivity.

Methods and systems for detection of antibiotic resistance

Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as-specific bacteriophage, allows detection of a specific microorganism, such asspp. and an indicator signal may be amplified to optimize assay sensitivity.

Methods and systems for the rapid detection ofusing infectious agents

Disclosed herein are methods for the production of mutant bacteriophages with altered host range. Additionally, disclosed herein are methods and systems for rapid detection of microorganisms such asspp. in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as-specific bacteriophage, allows detection of a specific microorganism, such asspp. and an indicator signal may be amplified to optimize assay sensitivity.

Methods for producing mutant bacteriophages for the detection of listeria

Disclosed herein are methods and systems for rapid detection of microorganisms such as bacteria in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene encoding one subunit of an indicator protein. The specificity of the bacteriophage allows detection of a particular bacteria of interest and an indicator signal may be amplified to optimize assay sensitivity.

Methods and systems for the rapid detection of bacteria using recombinant bacteriophage to express an indicator subunit

Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as CBA120, allows detection of a specific microorganism, such asO157:H7, and an indicator signal may be amplified to optimize assay sensitivity.

Disclosed herein are methods and systems for detection of a microorganism of interest using an amplification assay. The methods and systems utilize the specificity of binding agents, such as antibodies, to rapidly detect low levels of a specific microorganism present in a sample. In certain embodiments, antibodies are bound to a large number of the available binding sites on a microorganism.

Methods and systems for rapid detection of microorganisms using free antibodies

Disclosed are methods and systems for the isolation and detection of microbes from a sample. The use of binding agents for isolation of a microbe of interest from a sample are described. In certain embodiments, the methods use ribosome-based and/or bacteriophage-based amplification of the signal in detection of bacteria and other microorganisms. For example, embodiments of the present invention can achieve total amplification of at least 10,000 from a single infected cell.

Growing community of inventors

White Bear Township, MN, United States of America

Stephen Erickson