University of Michigan

Depuy Synthes Products, Inc.

Wisconsin Alumni Research Foundation

The Ohio State University

Tissue Regeneration Systems, Inc.

Stephen E Feinberg

William Leo Murphy

Scott J Hollister

James R Adox

Leenaporn Jongpaiboonkit

Francesco Migneco

Cynthia Marcelo

Arthur J Epstein

Mary-Ann Mycek

Frank LaMarca

Derek James Hansford

Chia-Ying Lin

William Lloyd

Blake J Roessler

Leng-Chun Chen

Shiuhyang Kuo

Hyungjin Myra Kim

Kenji Izumi

Yasushi Fujimori

Yanyin Yang

Frank Lamarca

Provided are biocompatible and implantable scaffolds for treating a tissue defect, such as a bone gap. The scaffolds can have a modular design comprising a tissue scaffold rack designed to accommodate one or more modules. Also provided are methods for fabrication and use of such scaffolds.

Modular tissue scaffolds

This disclosure provides a method of determining the viability of at least one cell via quantification of nicotinamide adenine dinucleotide (phosphate) (NAD(P)H), flavin adenine dinucleotide (FAD), and/or collagen. The method includes the steps of contacting the at least one cell with light having a wavelength of from 700 to 900 nm, using two photon excitation, or from 335 to 400 nm, using one photon excitation, to induce an optical response from the NAD(P)H, FAD, and/or collagen and measuring the optical response. The method also includes the step of quantifying one or more of an amount, spatial localization, and/or time-dependent response of the NAD(P)H, FAD, and/or collagen utilizing the optical response.

Method of determining the viability of at least one cell

In accordance with some preferred embodiments, without limitation, the present invention comprises compositions, methods and systems for preparation of stem-cell enriched cell populations from sources of biological materials by sorting cell types in relation to size.

Compositions, methods and systems for preparation of a stem cell-enriched cell population

The present invention is generally directed to a method for regulating cellular and tissue physiology, a device for practicing the method, and a process for fabricating the device. In general the process comprises the steps of providing at least one patterned electrode, providing a least one cell, placing the at least one electrode in electrical communication with the at least one cell, and applying a voltage to the electrode thereby delivering an effective amount of a patterned electric field or current thus regulating the physiology and/or growth of the at least one cell.

Electrical stimulation of cell and tissue growth with two-and three-dimensionally patterned electrodes

Methods for the engineering and preparation of intervertebral disc repair scaffolds and articulating joint repair scaffolds are disclosed. The methodology utilizes either magnetic resonance images or combined magnetic resonance and computed tomography images as a template for creating either the intervertebral scaffold or the joint repair scaffold (e.g., osteochondral scaffold) with fixation to the underlying bone. The disc scaffold design may include an outer annulus that may contain desired structures and a central nucleus pulposus region that could either contain a designed microstructure or a contained hydrogel. The osteochondral scaffold may include a bone compartment interface with a cartilage compartment. The bone compartment may interface with a cutout portion of the bone through fixation components. Different microstructure designs may be created for the bone and cartilage compartment to represent desired mechanical and mass transport properties. The scaffolds are designed with a microstructure that controls elastic and permeability property distribution within the scaffold.

Engineered scaffolds for intervertebral disc repair and regeneration and for articulating joint repair and regeneration

The present invention discloses a composite made of a layer of epithelial cells cultured together with a dermal matrix in a culture medium, which is free of BPE. The dermal matrix is a substantially non-immunogenic human dermis. Also disclosed is an enhanced composite where a biological agent has been introduced into the epithelial cells. The invention also comprises methods of making and implanting both the composite and the enhanced composite in an animal. A method of treating disease or injury through the use of the disclosed composites is also part of the present invention.

Mucosal cell composites and methods

Growing community of inventors

Ann Arbor, MI, United States of America

Stephen E Feinberg