G.d. Searle & Company

Pharmacia Corporation

Monsanto Company

The Ohio State University

Stephen C Lee

John Patrick McKearn

Barbara Kure Klein

S Christopher Bauer

Maire Helena Caparon

Judith G Giri

Charles A McWherter

Yiqing Feng

Nicholas R Staten

Linda L Zurfluh

Charles M Baum

Neena L Summers

Peter O Olins

Ranjani V Parthasarathy

Paul Berger

Verne A Luckow

Leonard J Brillson

Rajani V Parthasarathy

Gregg A Hadley

Mark S Leusch

Wu Lu

Ronald P Pelletier

Disclosed are field effect transistor-based (FET-based) sensors for the rapid and accurate detection of analytes both in vivo and in vitro. The FET-based sensors can include a substrate, a channel disposed on the substrate, a source electrode and a drain electrode electrically connected to the channel, and a recognition element for an analyte of interest immobilized on the surface of the channel via a linking group. The distance between the recognition element and the channel can be configured such that association of the analyte of interest with the recognition element induces a change in the electrical properties of the channel. In this way, an analyte of interest can be detected by measuring a change in an electrical property of the channel. Also provided are devices, including probes and multi-well plates, incorporating the FET-based sensors.

Devices and methods for the rapid and accurate detection of analytes

Disclosed are novel multi-functional hematopoietic receptor agonist proteins, DNAs which encode the multi-functional hematopoietic receptor agonists proteins, methods of making the multi-functional hematopoietic receptor agonists proteins and methods of using the multi-functional hematopoietic receptor agonists proteins.

Fused G-CSF and IL-3 proteins and uses thereof

The present invention relates to site-specific conjugation of synthetic particles to proteins.

Site specific ligation of proteins to synthetic particles

Multi-functional hematopoietic fusion proteins between sequence

A method for producing infectious recombinant baculoviruses in bacteria is described. A novel baculovirus shuttle vector (bacmid) was constructed that contains a low-copy-number bacterial replicon, a selectable drug resistance marker, and a preferred attachment site for a site-specific bacterial transposon, inserted into a nonessential locus of the baculovirus genome. This shuttle vector can replicate in E. coli as a plasmid and is stably inherited and structurally stable after many generations of growth. Bacmid DNA isolated from E. coli is infectious when introduced into susceptible lepidopteran insect cells. DNA segments containing a viral promoter driving expression of a foreign gene in insect cells that are flanked by the left and right ends of the site-specific transposon can transpose to the attachment site in the bacmid propagated in E. coli when transposition functions are provided in trans by a helper plasmid. The foreign gene is expressed when the resulting composite bacmid is introduced into insect cells.

Method of producing recombinant eukaryotic viruses in bacteria

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St. Louis, MO, United States of America

Stephen C Lee