Pneumatic Scale Corporation

Other

Kendro Laboratory Products Gmbh

Hemasure, Inc.

Stephen B Kessler

T David Marro

Robert B Carr

Ascher H Shapiro

Sumith Ranil Wickramasinghe

Daniel S Sutton

Randal A Goffe

James L O'Connor

Stephen E Zale

Gary W Brown, Sr

William M Cox

An apparatus for separating cell suspension material into centrate and concentrate, includes a single use structure () releasably positioned in a cavity in a solid wall rotatable centrifuge bowl (). The bowl and portions of single use structure rotate about an axis (). A stationary inlet feed tube (), a centrate discharge tube () and a concentrate discharge tube () extend along the axis of the rotating single use structure. A centrate centripetal pump () is in fluid connection with the centrate discharge tube. A concentrate centripetal pump () is in fluid connection with the concentrate discharge tube. A controller () operates responsive to sensors () in respective centrate and concentrate discharge lines (), to control flow rates of a concentrate pump () and a centrate pump () to produce output flows of cell concentrate and generally cell free centrate.

Centrifuge system for separating cells in suspension

An apparatus for separating cell suspension material into centrate and concentrate, includes a single use structure () releasably positioned in a cavity in a solid wall rotatable centrifuge bowl (). The bowl and portions of single use structure rotate about an axis (). A stationary inlet feed tube (), a centrate discharge tube () and a concentrate discharge tube () extend along the axis of the rotating single use structure. A centrate centripetal pump () is in fluid connection with the centrate discharge tube. A concentrate centripetal pump () is in fluid connection with the concentrate discharge tube. At least one concentrate channel () and a concentrate centripetal pump chamber () have configurations in the structure that facilitate the flow of cell concentrate.

A method and apparatus for separating cell suspension into centrate and concentrate includes a solid wall rotatable centrifuge bowl (). A single use rotatable core () is positioned in the bowl and bounds a cavity () which serves as a separation chamber. The bowl rotates about an axis (). An inlet tube () and an outlet tube () extend along the axis in the bowl cavity. The centripetal pump () in the cavity is in connection with the outlet tube. The centrate discharge pump () is in connection with the outlet tube. A pressure damping reservoir () is fluidly connected between the outlet tube and the discharge pump. At least one control circuit () is operative to control the apparatus to maintain a positive pressure above atmospheric pressure within the cavity, so as to maintain the cell suspension away from at least one seal () that bounds the interior of the cavity.

Centrifuge system including a control circuit that controls positive back pressure within the centrifuge core

A method and apparatus for cell harvest of production scale quantities of cell cultures using single use components comprising a flexible membrane mounted on a rigid frame and is supported within a multiple use rigid centrifuge bowl, such single use components including a core with an increased diameter and an internal truncated cone shape in order to permit the system to maintain a sufficiently high angular velocity to create a settling velocity suited to efficiently processing highly concentrated cell culture streams. Features which minimize feed turbidity, and others which permit the continuous or semi-continuous discharge of cell concentrate, increase the overall production rate over the rate which can be achieved using current intermittent processing methods for large cell culture volumes. Injection of a diluent during the cell concentrate removal process permits more complete removal of viscous cell concentrates.

Single use centrifuge system for highly concentrated and/or turbid feeds

A chamber or series of chambers is formed from layers of nested shells and used to manipulate a work product within the chambers. The shells are formed from highly ordered material structures, such as superconductors and metamaterials, and the work product is manipulated using energy that is directed from the outside of the chamber, through the nested shells and into the chamber. The nested shells have an open position and a closed position or have a bridge through which a working material can be passed from one set of nested shells to another set of nested shells. The superconducting shells can be type-1 or type-2, and the metamaterial shells can include any combination of a frequency agile metamaterial, a split ring resonator, an artificial structure of a wire medium, a unit cell of an artificial magnetic metamaterial, metamaterial superlattices and any combination thereof, or other highly ordered composite metamaterials.

Metamaterial spheric alignment mechanism

A chamber for manipulating a work product is formed in layers as a series of nested shells. The shells have an outer structural casing and an electromagnetic shield that surrounds a superconducting shell. The superconducting shell is immersed in a cryogenic coolant contained in a reservoir. The work product is further manipulated using kinetic energy and electromagnetic energy.

Spheric alignment mechanism

A chamber or series of chambers for manipulating a work product is formed in layers as a series of nested shells. The shells have an outer structural casing and an electromagnetic shield that surrounds a superconducting shell. The superconducting shell is either room temperature or immersed in a cryogenic coolant contained in a reservoir. The work product is further manipulated using kinetic energy to move it through electromagnetic field amplifiers to facilitate energy release for power generation or motive propulsion.

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Princeton, MA, United States of America

Stephen B Kessler