Indigo Medical, Inc.

Endovasix, Inc.

Eve Biomedical, Inc.

Stephanos Papademetriou

Victor C Esch

William James Evans

Kelly Cox

Daren L Stewart

Thomas Henning

Yefim Gelfond

Theofilos Kosteroglou

Provided herein are nanostructure-based sequencing methods and systems. Such methods and systems include contacting an immobilized RNA polymerase with a double-stranded target nucleic acid molecule under sequencing conditions, where the sequencing conditions include the presence of four nucleoside triphosphates, where one of the nucleoside triphosphates is present in a rate-limiting amount; detecting the movement of the target nucleic acid molecule and/or one or more nascent strand(s) through, on or over a nanostructure; repeating the contacting and detecting steps a plurality of times; and determining the sequence of the target nucleic acid molecule based, sequentially, on the presence or absence of a change in the movement in the presence of the at least one nucleoside triphosphate.

Methods and compositions for nanostructure-based nucleic acid sequencing

An apparatus, including a light detecting means, determines the actual position of an optical fiber array having one or more, but fewer than all, short fibers by detecting light transmitted through the short fiber(s) as a laser beam is horizontally and/or vertically scanned across the proximal fiber end(s). The fiber array is attached to an alignment block that facilitates delivery of the array to an accurate positioning means comprising two substantially parallel dowels.

Apparatus and method for aligning an energy beam

A fiber optic radiation transmission system is provided in which an optical fiber and a radiation source are connected to establish optical communication therebetween. Additionally, a connecting system is provided for connecting an optical fiber and a radiation source such that optical communication is established therebetween. The systems include a connector which carries the optical fiber therein. The connector is extendable within the system toward the radiation source to bring the optical fiber into optical communication with the radiation source. More particularly, the systems are configured in such a way that when the connector is extended sufficiently within the system, it seats the optical fiber relative to the radiation source under bias, thereby establishing a reliable optical connection. Once the optical connection is thus established, the connector engages a connection port associated with the radiation source to secure the system in a fully connected configuration appropriate for use. Thus, in a method of using such a system, the user simply extends the connector until the connector engages the connection port. The present invention thus provides efficient and reliable systems in which optical communication is established between an optical fiber and a radiation source.

Methods of connecting an optical fiber and methods of providing

Connection apparatus with optical fiber coding and detection means or

Fiber optic radiation transmisson system connector for an optical fiber

Growing community of inventors

Sunnyvale, CA, United States of America

Stephanos Papademetriou