Roche Diagnostics Operations, Inc.

Stephanie Ronning

Johann-Peter Thalhofer

Claus S Schneider

Frank Geipel

Stephan Glaser

Hellmut Eckstein

Bernhard Rexer

Rainer Muller

Michael Ratka

Claudia Giessel

Bernhard Suppmann

Helmut Schoen

Rainer Müller

The present invention concerns a method for producing recombinant trypsin from porcine pancreas inwhich is soluble and secreted into the culture medium, whereby expression at pH 3.0-4.0 substantially prevents activation of trypsinogen to &#x3b2;-trypsin and autolysis of &#x3b2;-trypsin by &#x3b1;-trypsin into &#x3b5;-trypsin and from there into inactive peptides.

Method for producing recombinant trypsin

A method is provided for the production of a bovine pancreatic protein with a specific desoxyribonuclease activity of at least 6,000 units per mg of protein. Methylotrophic yeast is used as a heterologous host organism. The bovine pancreatic protein is secreted into the growth medium from which it is purified.

Recombinant bovine pancreatic desoxyribonuclease I with high specific activity

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Penzberg, Germany

Stephanie Ronning