Dow Agrosciences LLC

Agrigenetics, Inc.

Doe Agrosciences LLC

Stephanie L Burton

Aaron Todd Woosley

Thomas Meade

Joel Jay Sheets

Kenneth Edwin Narva

Nicholas P Storer

Todd P Glancy

Sarah E Worden

Diaa Alabed

Sek Yee Tan

Karen C McLaughlin

Donald J Merlo

Timothy Denver Hey

Joel J Sheets

Aaron T Woosley

Ignacio Mario Larrinua

Qihua C Cai

Dong Liu

Haley Jo Moon

Kenneth Narva

Donald Joseph Merio

Karen C Mclaughlin

Kenneth E Narva

The subject invention relates in part to the surprising discovery that CryDa is active against corn earworm (CEW),(Boddie). Methods for using CryDa in transgenic plants to prevent serious crop damage is described. Leaf and silk bioassays using transgenic maize expressing full length, core toxin region or chimeric CryDa demonstrated good insect protection against CEW larvae damage.

Cry1D for controlling corn earworm

The subject invention relates in part to the surprising discovery that Cry1Da is active against corn earworm (CEW),(Boddie). Methods for using Cry1Da in transgenic plants to prevent serious crop damage is described. Leaf and silk bioassays using transgenic maize expressing full length, core toxin region or chimeric Cry1Da demonstrated good insect protection against CEW larvae damage.

The subject invention includes methods and plants for controlling European corn borer, said plants comprising a Cry1Ab insecticidal protein and a DIG-3 insecticidal protein to delay or prevent development of resistance by the insect.

Use of DIG3 insecticidal crystal protein in combination with cry1Ab for management of resistance in european cornborer

CRY1D for controlling corn earworm

The subject invention includes methods and plants for controlling fall armyworm lepidopteran insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Ca insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

Use of Cry1Da in combination with Cry1Ca for management of resistant insects

The subject invention includes methods and plants for controlling European corn borer and/or fall armyworm insects, said plants comprising a Cry1Ab insecticidal protein and a Cry1Be insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

Use of Cry1Ab in combination with Cry1Be for management of resistant insects

The subject invention includes methods and plants for controlling lepidopteran insects. The plants comprise a Cry1Fa insecticidal protein and a Cry1Ca insecticidal protein, in combination, to delay or prevent development of resistance by the insect(s).

Combined use of CRY1Ca and CRY1Fa proteins for insect resistance management

The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both(FAW) and(ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins/genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.

Insect resistance management with combinations of Cry1Be and Cry1F proteins

The subject invention includes methods and plants for controlling fall army worm insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Be insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

Use of Cry1Da in combination with Cry1Be for management of resistant insects

The present invention includes modified, insecticidal B.t. Cry1Ca proteins, including the proteins designated herein as DIG-109 and DIG-152, as well as variants of DIG-109 and DIG-152, nucleic acids encoding these proteins, methods of controlling pests using the proteins, methods of producing the proteins in transgenic host cells, and transgenic plants that produce the proteins. The DIG-109 and DIG-152 proteins comprise chimeric peptides composed of a core toxin segment of B.t. Cry1Ca and a Cry1Ab protoxin segment. Insecticidally active variants of the DIG-109 and DIG-152 proteins are also described.

Modified Cry1Ca insecticial Cry proteins

The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising Cry1Ca insecticidal protein and a Cry1Ab insecticidal protein in combination to delay or prevent development of resistance by the insects.

Combined use of Cry1Ca and Cry1Ab proteins for insect resistance management

The subject invention includes methods and plants for controlling lepidopteran insects, said plants comprising a Vip3Ab insecticidal protein in combination with a Cry1Ab protein to delay or prevent development of resistance by the insect(s)&#x2014;particularly corn earworm.

Combined use of Vip3Ab and Cry1Ab for management of resistant insects

The subject invention relates to insecticidal toxin complex ('TC') fusion proteins and to polynucleotides that encode these fusion proteins. In some embodiments, the invention provides a fusion protein comprising a Class A protein, a Class B protein, and a Class C TC protein fused together to form a single protein. In some other embodiments, the invention provides a fusion protein comprising a Class B and a Class C TC proteins fused together. In the latter embodiments, the BC or CB fusion protein can be used to enhance or potentiate the anti-insect activity of a 'Toxin A' or Class A protein. The subject invention also includes plants, cells (bacterial and plant cells for example), and seeds that comprise the polynucleotides. The subject invention also includes methods of controlling pests (preferably insects and other plant pests) with fusion proteins of the subject invention.

Growing community of inventors

Indianpolis, IN, United States of America

Stephanie L Burton