Genomatica, Inc.

Persephone Biosciences, Inc.

Braskem S.a.

Stephanie June Culler

Kristian Luis Jerman

Shaun Adam Singer

Priti Pharkya

Robin E Osterhout

Mark Joseph Burk

Stephen J Van Dien

Iuri Estrada Gouvea

Robert Haselbeck

Daniel Johannes Koch

Lucas Pedersen Parizzi

Kevin G Hoff

Harish Nagarajan

Mateus Schreiner Garcez Lopes

Nicholas Eakley

Erik C Ralph

Robert J Haselbeck

Anandh Swaminathan

Hirokazu Sato

Stephen Van Dien

Collection container

In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.

Vinylisomerase-dehydratases, alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases and methods for making and using them

Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.

Engineered microorgansims and methods for improved crotyl alcohol production

The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.

Microorganisms for producing 4C-5C compounds with unsaturation and methods related thereto

Growing community of inventors

Del Mar, CA, United States of America

Stephanie June Culler