Forschungszentrum Julich Gmbh

Forschungszentrum Juelich Gmbh

Rhein Biotech

Stephanie Bringer-Meyer

Hermann Sahm

Lothar Eggeling

Michael Bott

Stephan Binder

Georg Schendzielorz

Solvej Siedler

Ralf Klasen

Cornelies Petrus Hollenberg

An NADP(H) nanosensor has i) a nucleic acid sequence to which a regulator is capable of binding, wherein the oxidation state of the regulator depends on the NADP(H) availability; ii) a promoter sequence following the nucleic acid sequence i), to which an RNA polymerase is capable of binding, wherein the affinity of the RNA polymerase for the promoter sequence is influenced by the oxidation state of the regulator; iii) a nucleic acid sequence which is under the control of the promoter sequence ii) and which codes for an autofluorescent protein. The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

Sensor for NADP (H) and development of alcohol dehydrogenases

The present invention relates to an NADP(H) nanosensor comprising The present invention also relates to a cell, a method for isolating genes which code for NADP(H)-dependent enzymes, and the use of an NADP(H) nanosensor.

A method is disclosed for preparing 5-ketogluconate, which comprises the steps of:

Microbiological method of making 5-ketogluconate

A process is disclosed for obtaining sorbitol and gluconic acid, or gluconate, by fermentation in aqueous glucose/fructose mixtures. Zymomonas mobilis cells are used which have been permeabilized by the freeze technique and which have preferably been obtained by freezing cell centrifugates of pH 6 to 7 at about -20.degree. C., and thawing at room temperature. Cell concentrations of 20 to 60 g of cell dry matter of permeabilized cells/l are preferably used for the fermentation. By using cells which have been permeabilized by freezing and thawing, a considerably higher conversion rate of glucose/fructose into sorbitol and gluconic acid, or gluconate, is achieved.

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Julich, Germany

Stephanie Bringer-Meyer